tetrasodium 4-{4-[(9,10-dioxo-4-{[4-(2-sulfophenoxy)phenyl]amino}-9,10-dihydroanthracen-1-yl)amino]phenoxy}benzene-1-sulfonate 4-{4-[(9,10-dioxo-4-{[4-(4-sulfophenoxy)phenyl]amino}-9,10-dihydroanthracen-1-yl)amino]phenoxy}benzene-1-sulfonate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification Code: FAT 21030/D
Batch No.: 16.36
Appearance: black solid
Purity: 67% AS
Solubility (in water): 80 g/L
Received: 05/11/93
Storage: room temperature
Stability: 00/12/98

Analytical monitoring:

yes

Details on sampling:

Sampling for analysis:
Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 72 hours exposure and kept at -18 to -22 °C until analysis.

Vehicle:

no

Details on test solutions:

Stock solution: 50.4 mg test substance were mixed and made up to 1000 mL with water.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

- Strain: Green algae (Scenedesmus subspicatus)
- Origin: Collection of algae cultures, Pflanzenphysiologisches Institut, University, Nikolausberger Weg 18, D-3400 Göttingen, Germany
- Initial cell desity: 11000 cells/mL
- Preculture: 3 days under test conditions

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

none

Test temperature:

23 +/- 1 °C

pH:

7.8-8.2

Nominal and measured concentrations:

Nominal: 2, 4.4, 9.6, 21 and 46 mg/L
Measured start: 2, 4.4, 9.8, 21.6 and 47.6 mg/L
Measured end: 1.6, 3.0 5.1, 12.3 and 30.9 mg/L

Details on test conditions:

- Vessels 100 ml Erlenmeyer flasks, stoppered with aluminium caps, on Lab-shaker, 50 ml test solution per flask.
- Temperature 23 +/- 1 °C
- Lighting: Continuous illumination, cold white fluorescent light, ca. 8000 lux.

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

9.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 7.1-12.8mg/l

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

5.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Results of analytical determination:

Blank (Start) 07.03.94; Content of test article in Daphnia water: <0.8 mg/L

Blank (End) 10.03.94; Content of test article in Daphnia water: <0.8 mg/L

2.0 mg/L (Start) 07.03.94; Content of test article in Daphnia water: 2.0 mg/L

2.0 mg/L (End) 10.03.94; Content of test article in Daphnia water: 1.6 mg/L

4.4 mg/L (Start) 07.03.94; Content of test article in Daphnia water: 4.4 mg/L

4.4 mg/L (End) 10.03.94; Content of test article in Daphnia water: 3.0 mg/L

9.6 mg/L (Start) 07.03.94; Content of test article in Daphnia water: 9.8 mg/L

9.6 mg/L (End) 10.03.94; Content of test article in Daphnia water: 5.1 mg/L

21 mg/L (Start) 07.03.94; Content of test article in Daphnia water: 21.6 mg/L

21 mg/L (End) 10.03.94; Content of test article in Daphnia water: 12.3 mg/L

46 mg/L (Start) 07.03.94; Content of test article in Daphnia water: 47.6 mg/L

46 mg/L (End) 10.03.94; Content of test article in Daphnia water: 30.9 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The 72h EC50 (growth rate) was found to be 9.6 mg/L. NOEC (growth rate) was found to be 5.1 mg/L.

Executive summary:

A study was performed to determine the toxicity of the test substance (FAT 21030/D) on algae (Scenedesmus subspicatus) according to Guideline 92/69/EEC C.3 (GLP). The duration of the experiment was 72 hours.

The test was done at following concentrations:

Nominal: 2, 4.4, 9.6, 21 and 46 mg/L

Measured start: 2, 4.4, 9.8, 21.6 and 47.6 mg/L

Results: In conclusion, the 72-hour EC50 (growth rate) was found to be 9.6 mg/L. NOEC (growth rate) was found to be 5.1 mg/L.

Description of key information

A study was performed to determine the toxicity of the test substance (FAT 21030/D) on algae (Scenedesmus subspicatus) according to Guideline 92/69/EEC C.3 (GLP). The duration of the experiment was 72 hours.

The test was done at following concentrations:

Nominal: 2, 4.4, 9.6, 21 and 46 mg/L

Measured start: 2, 4.4, 9.8, 21.6 and 47.6 mg/L

Results: In conclusion, the 72h EC50 (growth rate) was found to be 9.6 mg/L. NOEC (growth rate) was found to be 5.1 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:

9.6 mg/L

EC10 or NOEC for freshwater algae:

5.1 mg/L