4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with biphenyl-4-ol

Endpoint summary

Additional information

Four short-term toxicity tests and one chronic study were conducted to evaluate the toxicity of the test substance.

Two fish short-term toxicity test were conducted. Both test result indicated 96h-LC50 is more than the water solubility of the test substance (<0.025 mg/L) greatly. One fish acute toxicity test was conducted according to OECD TG 203 with GLP, one test concentration of 102.4 mg/L and two controls were applied, including three sorts of adjuvant controls. During the whole test period, no death occurred at the test concentration of 102.4 mg/L and control groups. The test was valid based on the criterion in OECD test guideline. Thus, 96h-LC50 of the test article is greater than 102.4 mg/L. The other acute fish toxicity test was conducted according to OECD TG 203 was well in semi-static system, only one concentration of 20 mg/L and control groups were applied. And, the 96-hour LC50 of the test substance was >20 mg/L.

The acute toxicity to daphnia was conducted according to OECD guideline No. 202, twenty daphnids per concentration (5 per replicate, 4 replicates) were exposed to a control and a WAF prepared at a loading rate of 100 mg/l. Additionally, 10 daphnids per concentration were exposed to dilutions containing 0.1, 1.0 and 10% of the WAF prepared at a loading rate of 100 mg/l. The test duration was 48 hours and samples for analytical confirmation of test concentrations were taken at the start and at the end of the test. The actual concentration in the sample taken from the WAF prepared at 100 mg/l at the start of the test was 0.76 mg/l. The measured concentration decreased to 0.13 mg/l at the end of the exposure period. Based on these results, the average exposure concentration was estimated to correspond to 0.31 mg/l.Thus, 48h-EC50 is greater than 0.31 mg/L (measured concentration) under the test conditions, which was higher than the maximum water solubility (< 0.025 mg/l). Due to the very low solubility of Epikote P in water (< 0.025 mg/l), concentration levels that might be toxic for Daphnia magna could not be reached.

The algae growth inhibition test with the test substance was conducted according to OECD TG 201 with GLP, one control and a loading rate of 100 mg/L groups were applied. The actual test concentration in the sample taken from the WAF prepared at a loading rate of 100 mg/l was 0.72 mg/l at the start of the test and decreased to 0.13 mg/l after 24 hours of exposure. At the end of the test the measured concentration was 0.32 mg/l. Based on these results, the Time Weight Average concentration was 0.23 mg/l. The study met the acceptability criteria prescribed by the protocol and was considered valid. No reduction of growth rate or inhibition of yield was recorded at any of the concentrations of Epikote P tested. Thus, the EC50 for growth rate reduction (ERC50: 0-72h) and the EC50 for yield inhibition (EYC50: 0-72h) were both exceed a loading rate of 100 mg/l, corresponding to a TWA concentration of 0.23 mg/l. The NOEC for growth rate reduction and yield inhibition equalled a loading rate of 100 mg/l, corresponding to a TWA concentration of 0.23 mg/l. Due to the very low solubility of Epikote P in water (< 0.025 mg/l), concentration levels that might be toxic for algae could not be reached.

A study was conducted to determine the effects of the test substance on the reproductive capacity of Daphnia magna according to OECD Guideline 211 and OECD Guidance document 23. The reproduction test was done as a limit test based on the results of a preceding range-finding study. The reproduction test was performed in a semi-static system. The study duration was 21 d and the test solutions were renewed every 48 h. 3 out 20 parental daphnids in the control and 1 out of 20 daphnid died in the test group. On average, 92 offspring were produced per surviving daphnid in the control treatment whereas 101 offspring were counted on average in the treatment group. There was no delay in first reproduction in the test group in comparison to the control. There were a few insignificant recordings of immobile young and appearance of unhatched (aborted) eggs in the test group during the 21 -d test period. The mean body length of the parents exposed to the control and the test substance group were comparable. The concentrations measured in the freshly prepared test solutions ranged between 0.035 and 0.218 mg/L (average measured concentration of 0.068 mg/L) and were expected to be above the water solubility limit of the test substance (<0.025 mg/L). The study met the acceptability criteria prescribed by the study plan and was considered valid.Under the study conditions, the 21-d NOEC and EC10 of the test substance for reproduction in Daphnia magna was determined to be 0.068 mg/L (average measured concentration)

The influence of E-200 on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The study procedures described in this report were based on the OECD guideline No. 209, 1984. Two 1 litre test bottles were filled with 250 ml of test substance mixtures with initial loading rates of 200 mg/l. These mixtures were stirred in dark closed bottles for 24 hours. Subsequently, 16 ml synthetic sewage feed, 200 ml sludge and Milli-RO water up to 500 ml were added resulting in a loading rate of 100 mg/l. This loading rate was tested in duplicate. No (significant) inhibition of respiration rate of the sludge was recorded at a loading rate of 100 mg E-200 per litre. Hence, the EC50 of E-200 exceeded a loading rate of 100 mg/l (nominal concentrations). Thus, Epikote P was not toxic to waste water (activated sludge) bacteria at a loading rate of 100 mg/l ( nominal concentrations).

In summary, no aquatic toxicity has been observed at all 3 trophic levels and in sludge bacteria at water solubility and above. The substance is therefore considered to be non-toxic following exposure in the environment to algae, invertebratae and fish.