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EC number: 299-966-6 | CAS number: 93918-85-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
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- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
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- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 May 2001 to 07 May 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study was not conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- The study was performed with the strains TA 98 and TA 100 only
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Laurylmercaptobytyronitril
- IUPAC Name:
- Laurylmercaptobytyronitril
- Details on test material:
- - Name of test material (as cited in study report): Laurylmercaptobytyronitril
- Substance type: Mono-constituent
- Physical state: Liquid
- Analytical purity: 99%
- Purity test date:
- Lot/batch No.: P 5889001
- Expiration date of the lot/batch: 01 April, 2002
- Storage condition of test material: At room temperature
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 rat liver microsomal fraction
- Test concentrations with justification for top dose:
- 3, 10, 33, 100, 333, 1000, 2500, and 5000 ug/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethanol
- Justification for choice of solvent/vehicle: Solubility properties and its relative non-toxicity to the bacteria.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- This positive control was utilized in S. typhimurium strains TA 98 and TA 100 with metabolic activation.
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- This positive control was utilized in S. typhimurium strain TA 100 without metabolic activation.
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylene-diamine, 4-NOPD
- Remarks:
- This positive control was utilized in S. typhimurium strain TA 98 without metabolic activation.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: 4 hours
- Exposure duration: 48 hours
- Expression time (cells in growth medium): 52 hours
- Selection time (if incubation with a selection agent): 48 hours
- Fixation time (start of exposure up to fixation or harvest of cells): 48 hours
SELECTION AGENT (mutation assays): L-Histidine
NUMBER OF CELLS EVALUATED: All plates were evaluated for an increase in revertant colonies. - Evaluation criteria:
- A test item is considered positive if a dose related increase in the number of revertants or a biologically relevant increase for at least one test concentration is induced.
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: None
- Effects of osmolality: None
- Evaporation from medium: None
- Precipitation: No precipitation was observed at any dose.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Based on the results of the study, the test article is not mutagenic in a screening Ames Assay. - Executive summary:
The mutagenic potential of the test article was evaluated in a screening Bacterial Reverse Mutation Assay with S. typhimurium strains TA 98 and TA 100 in the presence and absence of a metabolic activation system (S9 mix). The study was not performed in compliance with GLP regulations. The test method was based on OECD 471 (1997) but only S. typhimurium strains TA 98 and TA 100 were utilized. The test article was dissolved in ethanol for dosing at 3, 10, 33, 100, 333, 1000, 2500, and 5000 ug/plate and all strains were tested in triplicate. Separate experiments were performed in the presence and absence of metabolic activation with S9 mix. Strain specific positive controls and vehicle controls were tested in parallel. No toxic effects were seen in the study. No reduction in the number of colonies occurred in the presence or absence of metabolic activation. No increase in the number of revertant colonies was seen in any strain in the presence or absence of metabolic activation. Controls performed as expected. Based on the results of the study, the test article is not mutagenic in the screening Ames Assay in the presence or absence of metabolic activation.
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