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EC number: 244-691-9 | CAS number: 21981-33-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study in accordance to OECD GL
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,1,1,3,3-pentachlorobutane
- EC Number:
- 244-691-9
- EC Name:
- 1,1,1,3,3-pentachlorobutane
- Cas Number:
- 21981-33-9
- Molecular formula:
- C4H5Cl5
- IUPAC Name:
- 1,1,1,3,3-pentachlorobutane
- Details on test material:
- - Name of test material (as cited in study report): 1,1,1,3,3-pentachlorobutane
- Molecular formula (if other than submission substance): same as submission substance
- Molecular weight (if other than submission substance): same as submission substance
- Smiles notation (if other than submission substance): same as submission substance
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: liquid
- Analytical purity: 90%
- Impurities (identity and concentrations):
- Purity test date: 09/09/99
- Lot/batch No.: 99350L08
- Expiration date of the lot/batch: 13/09/2000
- Stability under test conditions: stable
- Storage condition of test material: at room temeprature in the dark
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- Preliminary test (TA100 and WP2uvrA, +/- S9-mix): 3, 10, 33, 100, 333, 1000, 3330, 5000 ug/plate
Main test
see table 1
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: -S9-mix: Sodium azide (TA1535), 9-aminoacridine (TA1537), methylmethane sulfonate (TA100), Daunomycine (TA98), 4-nitroquinolone (WP2uvrA)/ +S9-mix: 2-aminoanthracene (all strains)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period:
- Exposure duration: 48 h
-
DETERMINATION OF CYTOTOXICITY
- Method: reduction of the bacterial background lawn, redction of revertant colonies - Evaluation criteria:
- number of revertant colonies induced by the substance is 2 times greater than the revertants induced by the negative control in any of the tetsted strains, either with or without metabolic activation. The positive response has to be reproducible in at least 1 independent experiment.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Genreally observed at > 333 ug/plate and >1000 ug/plate in all the S. tiphymurium strains with and without metabolic activation, respectively.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- Positive results observed in the preliminary test and in the main test (experiment 2). Negative responses observed in experiments 3 and 4.
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Only minimal toxicity observed at > 1000 ug/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Only minimal toxicity observed at 5000 ug/plate.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Positive results were observed for WP2uvrA in the absence of S9-mix in the preliminary test and in the main test (experiment 2). The experiment was repeated by preventing the test substance evaporation (experiments 3 and 4). Negative results were observed in the repeated experiments
TEST-SPECIFIC CONFOUNDING FACTORS
- Evaporation from medium: In experiments 3 and 4 the plates were sealedwith parafilm to avoid possible evaporation of the test substance
- Precipitation: precipitation of the test substance was observed at the start of the incubation. At the end of the incubation no precipitation was visible anymore.
COMPARISON WITH HISTORICAL CONTROL DATA:
The positive results observed for WP2uvrA in the preliminary test (up to 3.5 fold the number of revertant colonies observed in comparison to vehicle controls) was within the historical control data and was related to a low vehicle control data (average of 6 revertant colonies vs the historical control mean value of 12). In experiment 4 an increase up to 2 fold in the number of revertant colonies was observed for 1,1,1,3,3,-pentafluorobutane in comparison to vehicle control. Also in this case the increase was within the mean historical control value.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Overall the substance was judged as not mutagenic in all the tested strains both in the presence and in the absence of metabolic activation.
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