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As the registered substance PR 254 is highly insoluble in water, no study investigating acute aquatic toxicity is required according to REACH Annexes VII and VIII.

However, reliable studies are available from a NONS file provided by ECHA. According to these studies the LC50 to fish has been determined as 100 mg/L. Regarding the short-term toxicity to aquatic invertebrates, an EC100 of > 100 mg/L was indicated.

Furthermore, a long-term study with daphnis magna did not show any effects up to the maximum water solubility.