2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-3 (Acute inhalation toxicity)

GLP compliance:

yes

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

EC Number:

200-076-7

EC Name:

2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Cas Number:

51-03-6

Molecular formula:

C19H30O5

IUPAC Name:

2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

clean air

Details on inhalation exposure:

Piperonyl Butoxide was aerosolized and diffused by an air atomizing nozzle (Spraying Systems Company, ¼ inch JCO-SS nozzle with a #1A set-up)

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Nominal concentration a.i. 28 g/m³
Analytical concentration a.i. 5.9 mg/L

No. of animals per sex per dose:

10

Control animals:

yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.9 mg/L air

Exp. duration:

4 h

Mortality:

All animals survived the duration of the study.

Clinical signs:

other: During exposure nasal discharge, excessive salivation, eye closure and decreased activity were noted. On day one and during the first week excessive lacrimation and salivation nasal discharge and laboured breathing were noted. Most of these responses abat

Body weight:

Slight or no weight losses were observed on the day following exposure. This was considered a minimal response to exposure. Generally normal weight gains were seen thereafter.

Gross pathology:

Red foci were observed in the lungs of 2/5 female. The toxicologic significance of this finding on the basis of gross examination only is equivocal.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The mean analytical exposure concentrations was 5.9 g/m³ resulted in no mortalities. Treatment related decreases in body weight were observed, however, recovery occurred by day 3 and all surviving animals were well in excess of their pre-exposure body weight by day 5.
Clinical signs included secretory responses during exposure. Respiratory and secretory observations seen following exposure abated within a week afterwards.
Post mortem findings were also considered unremarkable.
The LC50 was found to be greater than 5.9 g/m³. EPA Category IV is determined for the acute inhalation toxicity of Piperonyl Butoxide.

Executive summary:

Ten of ten rats receiving a single four hour exposure to 5.9 mg/L Piperonyl Butoxide as a liquid aerosol, survived the exposure and subsequent 14day post exposure observation period. signs of treatment inclueded secretory responses during the exposure. respiratory and secretory responses were also seen following the exposure but generally abated within a week afterwards.

A minimal effect upon body weight was produced by treatment. Gross postmortem observations were also considered unremarkable.