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EC number: 214-770-2 | CAS number: 1193-21-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Screening study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- no
- Remarks:
- Screening-test
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4,6-dichloropyrimidine
- EC Number:
- 214-770-2
- EC Name:
- 4,6-dichloropyrimidine
- Cas Number:
- 1193-21-1
- Molecular formula:
- C4H2Cl2N2
- IUPAC Name:
- 4,6-dichloropyrimidine
Constituent 1
Method
- Target gene:
- Histidine gene
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 1513, TA 100, TA 1537, TA 98, TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9-Mix
- Test concentrations with justification for top dose:
- 0, 16, 50, 160, 500, 1600, 5000 µg/plate with and without S9-Mix - Plate incorporation screen
0, 100, 200, 400, 800, 1600, 3200 µg/tube Preincubation test with and without S9-Mix - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- other: other: sodium azide, nitrofurantoin, 4-nitro-1,2-phenylene diamine, cumene hydroperoxide, 2-aminoanthracene
Results and discussion
Test results
- Species / strain:
- other: other: S. typhimurium TA 1535, TA 100, TA 1537, TA 98, TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: without metabolic activation 5000 mg/tube, with metabolc activation 3200 mg/tube preincubation test
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No indication of a bacteriotoxic effect of the test substance at doses of up to and including 1600 µg/plate. The total bacteria counts consistently produced results comparable to the negative controls, or differed only insignificantly. No inhibition of growth was noted as well. Higher doses had a strain-specific bacteriotoxic effect. Therefore they could only partly be used for assessment purposes.
In the plate incorporation screen a dose-related and biologically relevant increase in mutant counts over those of the negative controls (with and without S9 -mix) could not be detected. Results were confirmed by the results of the preincubation screen.
Summary of results
S9 -mix TA 1535 TA 100 TA 1537 TA 98 TA 102
without neg. neg. neg. neg. neg.
with neg neg. neg. neg. neg.
The positive controls increased mutant counts to well over those of the negative controls, demonstrated the system´s sensitivity and the activity of the S9 mix.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative non-mutagenic
The test substance was considered to be non-mutagenic without and with S9-mix in the plate incorporation as well as in the preincubation modification of the Salmonella/mircosome test. - Executive summary:
The test subtance was screened with one plate per dose using Salomonella/microsome plate incorporation test for point mutagenic effects in doses of up to and including 5000 µg/plate (strains: TA 1535, TA 100, TA 1537, TA 98, TA 102). The independent repeat was performed as preincubation for 20 minutes at 37 °C and doses up to and including 3200 µg per tube. Doses up to and including 1600 µg per plate did not cause any bacteriotoxic effects: Total bacteria counts remained unchanged and no inhibition of growth was observed. At higher doses the substance had a strain-specific bacteriotoxic effect, so that this range could only be used to a limited extent.
Evidence of mutagenic activity of the test substance was not seen. No biologically relevant increase in the mutant count, in comparison with the negative controls, was observed.
The positive controls had a marked mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding negative controls.
Therefore the test substance was considered to be non-mutagenic without and with S9-Mix in the plate incorporation as well as in the preincubation modification of the Salomonella/microsome test.
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