Reaction products of enzymatic esterification of linolenic acid and 1,2-octanediol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-hydroxyoctan-2-yl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12-dienoate; octane-1,2-diol

EC Number:

944-490-2

Molecular formula:

unknown

IUPAC Name:

1-hydroxyoctan-2-yl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12-dienoate; octane-1,2-diol

Test material form:

liquid

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species and strain: Daphnia magna
Source: Szent István University, Department of Aquaculture, 2100 Gödöllő, Páter Károly u. 1. – Hungary
Justification of strain: Daphnia magna is the standard species of the acute immobilisation test.
Number of animals: There were 20 animals in test and control groups respectively, divided into 4 replicates. (5 animals / replicate)
Age of the animals: They were less than 24 h old at the beginning of the test.

Study design

Total exposure duration:

48 h

Test conditions

Hardness:

The reconstituted water (ISO medium) had a total hardness of 246 mg/L (as CaCO3).

Test temperature:

The water temperature was measured at the start and at the end of each renewal period in each test vessel. The test temperature was in the range of 20.1 – 20.7°C measured in the test vessels.

pH:

The pH of the test solution was not adjusted and not varied by more than 1.5 units in any one test. The pH was measured at the start and at the end of each renewal period in each test vessel and was in the range of 7.44 – 7.84.

Dissolved oxygen:

The dissolved oxygen concentration was measured in each test vessel at the start and at the end of each renewal period and was in the range of 7.4 – 8.7 mg/L.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

other: ELR50

Effect conc.:

40.83 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

other: ELR100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOELR

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

other: ELR50

Effect conc.:

44.87 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

In conclusion, under the conditions of this study, the calculated endpoints for the effect of the Test Item were the following:

 

Table 4:Summary of endpoints

Parameter	ELR50 (mg/L nominal loading rates WAF)	ELR100 (mg/L nominal loading rates WAF)	NOELR (mg/L nominal loading rates WAF)	LOELR (mg/L nominal loading rates WAF)
24 hours	44.87	>100	25	50
95 % conf. limits	41.71 – 48.28	---	---	---
48 hours	40.83	>100	25	50
95 % conf. limits	37.98 – 43.89	---	---	---

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes