[No public or meaningful name is available]

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 2010 - February 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Guidelines for testing of chemicals, No. 211

Version / remarks:

China Environmental Science Press, 2004

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

Adopted at September 21st, 1998

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Cas Number:

890707-29-6

Molecular formula:

C10H11N3O

IUPAC Name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Samples were withdrawn for analysis after exposure intervals of 0 and 48 hours past each test medium renewal

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Age at study initiation: < 24 h
- Feeding during test: yes, once every day
- Food type: Dry powder of Scenedesmus sp.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

18-22°C

pH:

7-8

Nominal and measured concentrations:

Nominal concentrations: 0 (control), 1.2, 2.4, 4.8, 8.2, 10.8 and 13.4 mg/L; measured concentrations: 0.96, 2.19, 4.71, 7.92, 9.68 and 11.79 mg/L (average values)

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers, 100 mL
- Aeration: No
- Renewal rate of test solution: Test medium was renewed every 48 h
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium (OECD guideline 211, annex II)
- Intervals of water quality measurement: pH and oxygen concentration were measured in all test concentrations and in the control at beginning and end of the respective periods. Temperature was measured in the control every day.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light vs 8 h dark
- Light intensity: 1500-2500 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality of adults and number of young was checked daily .

Results and discussion

Effect concentrations

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

ca. 10.64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The nominal concentration of the test substance that led to 50% reduction of reproduction of Daphnia magna (EC50) was calculated to be 10.64 mg/L.

Executive summary:

The effects of the test substance on the reproduction of Daphnia Magna was investigated according to the OECD guideline 211.

Daphnids were exposed to nominal concentrations of 0 (control), 1.2, 2.4, 4.8, 8.2, 10.8 and 13.4 mg/L for 21 d under semi-static conditions.

The concentration of the test substance that led to 50% reduction of reproduction of Daphnia magna (EC50) was calculated to be 10.64 mg/L.