Registration Dossier
Registration Dossier
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EC number: 619-946-6 | CAS number: 890707-29-6
Ecotoxicological information
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- June 2011- August 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.4200 (Seed Germination/Root Elongation Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-Amino-5-cyano-N,3-dimethylbenzamide
- Cas Number:
- 890707-29-6
- Molecular formula:
- C10H11N3O
- IUPAC Name:
- 2-Amino-5-cyano-N,3-dimethylbenzamide
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil: Clean quartz sand was mixed well with test solution and divided into petri dishes
Test organisms
open allclose all
- Species:
- Lycopersicon esculentum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Tomato
- Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Cucumber
- Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Lettuce
- Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Soybean
- Species:
- Brassica oleracea var. capitata
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Cabbage
- Species:
- other: Citrullus lanatus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Watermellon
- Species:
- Lolium perenne
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Ryegrass
- Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Rape
- Species:
- Oryza sativa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Paddy
- Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Corn
Study design
- Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: Quartz sand
- Limit test:
- no
- Post exposure observation period:
- The post exposure observation period ends when at least 65% of the control seed has germinated and developed roots that were at least 20 cm long.
Test conditions
- Test temperature:
- 25°C ± 1°C
- Details on test conditions:
- TEST SYSTEM
- Test container: Petri dish 150 mm in diameter
- No. of seeds per container: 10
- No. of replicates per treatment group:
Limit test: one blank control group and one test group (saturated conc.), each group with one or three replicates.
Definitive test: one blank control group and seven/eight test groups, each group with 3 replicates
GROWTH CONDITIONS
- Photoperiod: Complete darkness
- Day/night temperatures: 25°C ± 1°C
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
At the end of the test, the total and average germination number of each group were checked and recorded; the developmental state of the seeds were observed and recorded; any abnormal seedling development of appearance such as lesions, enhanced toot growth (measured), discoloration, swelling, loss of turgor, etc. was reported.
TEST CONCENTRATIONS
Range finding/limit study:
- Test concentrations: 176.4 mg/L
- Results used to determine the conditions for the definitive study: Germination rates and inhibition rates on tomato, soybean, cabbage, ryegrass, rape, paddy and corn were <50% , and thereby no need to perform final/definitive tests on these species. - Nominal and measured concentrations:
- Cucumber seed:
0, 10.1, 16.3, 26.1, 41.9, 67.2, 107.4, 171,8 mg/L
Lettuce seed:
0, 6.4, 10.1, 16.3, 26.1, 41.9, 67.2, 107.4, 171.8 mg/L
Watermelon seed:
0, 6.0, 9.5, 15.3, 24.5, 39.4, 63.1, 100.9, 161.4 mg/L - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Species:
- other: Lycopersicon esculentum ; Glycine max ; Brassica oleracea ; Lolium perenne ; Brassica napus ; Oryza sativa ; Zea mays
- Duration:
- 20 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 176 other: mg/l
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- germination
- Remarks:
- and root elongation inhibition
- Remarks on result:
- other: Limit test
- Species:
- Cucumis sativus
- Duration:
- 20 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 134 other: mg/l
- 95% CI:
- >= 118 - <= 151
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: root elongation inhibition
- Remarks on result:
- other: Definitive test
- Species:
- Lactuca sativa
- Duration:
- 20 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 109 other: mg/l
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- germination
- Remarks on result:
- other: Definitive test
- Species:
- Lactuca sativa
- Duration:
- 20 d
- Dose descriptor:
- EC50
- Effect conc.:
- 76.3 other: mg/L
- 95% CI:
- >= 73.5 - <= 79.2
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: root elongation inhibition
- Remarks on result:
- other: Definitive test
- Species:
- other: Citrullus lanatus
- Duration:
- 20 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 180 other: mg/L
- 95% CI:
- >= 164 - <= 194
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: root elongation inhibition
- Remarks on result:
- other: Definitive test
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the test, the EC50 (germination and root elongation inhibition) for tomato, soybean, cabbage, ryegrass, rape, paddy and corn seeds were > 176.4 mg/l
EC50 for cucumber seed root elongation inhibition was 134 mg/l.
EC50 for lettuce seed germination was 109 mg/l while EC50 for lettuce seed root elongation inhibition was 76.3 mg/l.
EC50 for watermelon seed root elongation inhibition was 180 mg/l
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