4-trans-Methoxy-4-trans-propyl-[1,1-bicyclohexyl]

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2010-09-13 to 2010-11-02

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish

Qualifier:

according to guideline

Guideline:

other: METI Guideline: Concentration Test on Chemical Substances in Fish

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 23 and 28

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION: According to guideline.
- Chemical name of vehicle: TWEEN 80
- Concentration of vehicle in test medium: 0.08 and 0.008 mg/L; Dose 1: Test item: 0.02 mg/L, Vehicle: 0.08 mg/L; Dose 1: Test item: 0.002 mg/L, Vehicle: 0.008 mg/L

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Lot No. 100414
Weight: 3.10 +/- 0.24 g
Length: 7.10 +/- 0.20 cm

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

0

Hardness:

250 mg/L

Test temperature:

25.0 ° +/- 0.4 °C

pH:

7.0

Dissolved oxygen:

7.0 mg/L

Salinity:

According to guideline

Details on test conditions:

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 7, 14, 21, 23 and 28. The analytical values show that the nominal concentrations of 0.02 and 0.002 mg/L were maintained at about 100% using Tween 80, respectively. At days 0, 7, 14, 21, 23 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane. The extract was analysed with GC.Due to the low bioaccumulation potential, no depuration phase was required.

Nominal and measured concentrations:

Nominal concentration: 0.02 mg/L
Analytically measured concentrations: day 0: 0.0186 mg/L; day 7: 0.0208 mg/L; day 14: 0.0210 mg/L; day 21: 0.0206 mg/L; day 23: 0.0210 mg/L; day 28: 0.0207 mg/L
Nominal concentration 0.002 mg/L
Analytically measured concentrations: day 0: 0.00183 mg/L; day 7: 0.00208 mg/L; day 14: 0.00206 mg/L; day 21: 0.00203 mg/L; day 23: 0.00202 mg/L; day 28: 0.00207 mg/L

Details on estimation of bioconcentration:

according to guideline

Key result

Type:

BCF

Value:

476 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: analytical concentration

Remarks:

Conc.in environment / dose:0.02 mg/L

Key result

Type:

BCF

Value:

449 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: analytical concentration

Remarks:

Conc.in environment / dose:0.002 mg/L

Details on kinetic parameters:

steady state was reached within 14 days due to the low uptake, no depuration phase was necessary

Metabolites:

none

Details on results:

- Mortality of test organisms: no effects
- Behavioural abnormalities: no effects
- Observations on body length and weight: no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 476 and 449. The test material showed no relevant bioaccumulation (BCF < 2000).

Executive summary:

Purpose

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions.

Study Design

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.02 and 0.0002 mg/L were used in this study. Tween 80 was used as vehicle at concentrations of 0.08 and 0.008 mg/L. The exposure was maintained during the couse of the study at about 100% of the nominal concentrations. The concentrations have been verified analytically using a GC method with FID detection.

Results

No signs of toxicity were observed throughout the study. The analysis reveals steady state BCFs of 476 and 449. Due to the low bioaccumulation potential, no depuration phase was required.

Conclusion

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 476 and 449.

Description of key information

The test item had a BCF of 476 according to OECD TG 305 compliant GLP study. The test material showed no relevant bioaccumulation (BCF < 2000).

Key value for chemical safety assessment

BCF (aquatic species):

476 dimensionless

Additional information

Purpose

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions.

 

Study Design

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.02 and 0.0002 mg/L were used in this study. Tween 80 was used as vehicle at concentrations of 0.08 and 0.008 mg/L. The exposure was maintained during the couse of the study at about 100% of the nominal concentrations. The concentrations have been verified analytically using a GC method with FID detection.

 

Results

No signs of toxicity were observed throughout the study. The analysis reveals steady state BCFs of 476 and 449. Due to the low bioaccumulation potential, no depuration phase was required.

 

Conclusion

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 476 and 449.