Registration Dossier
Registration Dossier
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EC number: 277-155-8 | CAS number: 72968-81-1
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From April 06th to May 19th, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Justification for type of information:
- Justification for read-across is detailed in the report attached to the IUCLID section 13.
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Reference
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From April 06th to May 19th, 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Justification for type of information:
- Justification for read-across is detailed in the report attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Part I, May 12, 1981, adopted 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was dissolved in the test medium at a concentration of 100 mg/l (stock solution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Breeding: bred in the testing laboratories, under standardized conditions.
- Age at study initiation: age of less than 24 hours. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Test temperature:
- 21.5 °C
- pH:
- Start: 8.3 - 8.4
End: 8.3 - 8.4 - Dissolved oxygen:
- Start: 8.4 - 8.8 mg/l
End: 8.6 - 9.1 mg/l - Nominal and measured concentrations:
- 1, 10 and 100 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beakers, containing 20 ml of test medium.
- Aeration: test medium was aerated to an oxygen concentration of 8 mg/l.
- No. of organisms per vessel: 10 animal.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to the OECD guideline. The water used was bidlstilled water.
- Total hardness: 14 dH°.
- CaCl2 x 2H2O: 294 mg/l
- MgSO4 x 7H2O: 123 mg/l
- NaHCO3: 65 rng/l
- KCl: 5.8 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: the pH of the reconstituted water was abjusted to 8.2.
- Photoperiod: 16 hours of illumination.
- Light intensity: 1000 Lux.
EFFECT PARAMETERS MEASURED
Test evaluations were done according to the OECD guideline 202 by visual control of the mobility of the daphnids after 24 hours. Basect on the data, an estimate of the EC0, 50 and 100 was given. - Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The EC0 was identified in the range of 1 - 10 mg/l
- Validity criteria fulfilled:
- yes
- Remarks:
- in the control, not more than 10 % of the Daphnia have been immobilized or trapped at the surface of the water and dissolved oxygen concentration was ≥ 60 % of the air saturation
- Conclusions:
- EC50 (24h) > 100 mg/l (nominal)
- Executive summary:
The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202. The nominal test concentrations applied were 1, 10 and 100 mg/l. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 1 mg/l; at the nominal concentrations of 10 and 100 mg/l 10 % of total fish died after 24 hours of exposure. 10 % of mortality was also observed in one of the two the blank controls.
The EC0 was identified to be in the range of 1 - 10 mg/l.
Conclusion
EC50 (24h) > 100 mg/l (nominal)
Immobilization of Daphnia magna
| Conc. Of test article (mg/l) | N. of animals | N. of responses 24 hrs | % of responses 24 hrs |
| 0 | 10 | 1 | 10 |
| 0 | 10 | 0 | 0 |
| 1 | 10 | 0 | 0 |
| 1 | 10 | 0 | 0 |
| 10 | 10 | 0 | 0 |
| 10 | 10 | 1 | 10 |
| 100 | 10 | 1 | 10 |
| 100 | 10 | 1 | 10 |
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Part I, May 12, 1981, adopted 1984
- GLP compliance:
- no
Test material
- Reference substance name:
- Similar substance 01 of Acid Orange 051:1
- IUPAC Name:
- Similar substance 01 of Acid Orange 051:1
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was dissolved in the test medium at a concentration of 100 mg/l (stock solution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Breeding: bred in the testing laboratories, under standardized conditions.
- Age at study initiation: age of less than 24 hours.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Test temperature:
- 21.5 °C
- pH:
- Start: 8.3 - 8.4
End: 8.3 - 8.4 - Dissolved oxygen:
- Start: 8.4 - 8.8 mg/l
End: 8.6 - 9.1 mg/l - Nominal and measured concentrations:
- 1, 10 and 100 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beakers, containing 20 ml of test medium.
- Aeration: test medium was aerated to an oxygen concentration of 8 mg/l.
- No. of organisms per vessel: 10 animal.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to the OECD guideline. The water used was bidlstilled water.
- Total hardness: 14 dH°.
- CaCl2 x 2H2O: 294 mg/l
- MgSO4 x 7H2O: 123 mg/l
- NaHCO3: 65 rng/l
- KCl: 5.8 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: the pH of the reconstituted water was abjusted to 8.2.
- Photoperiod: 16 hours of illumination.
- Light intensity: 1000 Lux.
EFFECT PARAMETERS MEASURED
Test evaluations were done according to the OECD guideline 202 by visual control of the mobility of the daphnids after 24 hours. Basect on the data, an estimate of the EC0, 50 and 100 was given.
Results and discussion
Effect concentrations
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The EC0 was identified in the range of 1 - 10 mg/l
Any other information on results incl. tables
Immobilization of Daphnia magna
| Conc. Of test article (mg/l) | N. of animals | N. of responses 24 hrs | % of responses 24 hrs |
| 0 | 10 | 1 | 10 |
| 0 | 10 | 0 | 0 |
| 1 | 10 | 0 | 0 |
| 1 | 10 | 0 | 0 |
| 10 | 10 | 0 | 0 |
| 10 | 10 | 1 | 10 |
| 100 | 10 | 1 | 10 |
| 100 | 10 | 1 | 10 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- in the control, not more than 10 % of the Daphnia have been immobilized or trapped at the surface of the water and dissolved oxygen concentration was ≥ 60 % of the air saturation
- Conclusions:
- EC50 (24h) > 100 mg/l (nominal)
- Executive summary:
The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202. The nominal test concentrations applied were 1, 10 and 100 mg/l. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 1 mg/l; at the nominal concentrations of 10 and 100 mg/l 10 % of total fish died after 24 hours of exposure. 10 % of mortality was also observed in one of the two the blank controls.
The EC0 was identified to be in the range of 1 - 10 mg/l.
Conclusion
EC50 (24h) > 100 mg/l (nominal)
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