Trisodium 2-[[6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)methylamino]-1-hydroxy-3-sulphonato-2-naphthyl]azo]naphthalene-1,5-disulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

BOD value

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-15

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

5 h

Details on study design:

None

Preliminary study:

None

Test performance:

None

Details on results:

None

Key result

Parameter:

BOD5

Value:

2.6 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

no

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD of the test substance FAT 40170 is: 2.6 mg O2/g.

Executive summary:

A study was performed to determine the chemical oxygen demand of FAT 40170 according to CIBA method A-15. Based on the findings from the study, the COD of FAT 40170 is 2.6 mg O2/g.

Taking the results of the corresponding COD determination into account the calculated BOD5/COD quotient is equal to zero, therefore the substance is considered to be not biodegradeable at all.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

BOD value

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-15

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

5 h

Details on study design:

None

Preliminary study:

None

Test performance:

None

Details on results:

None

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

no

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD of the test substance FAT 40170 is: 0.0 mg O2/g.

Executive summary:

A study was performed to determine the chemical oxygen demand of FAT 40170 according to CIBA method A-15. Based on the findings from the study, the BOD of FAT 40170 is 0.0 mg O2/g. Taking the results of the corresponding COD determination into account the calculated BOD5/COD quotient is equal to zero, therefore the substance is considered to be not biodegradeable at all.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

COD value

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-22

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Details on study design:

None

Preliminary study:

None

Test performance:

None

Details on results:

None

Key result

Parameter:

COD

Value:

221.1 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

no

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The COD of the test substance FAT 40170 is: 221.1 mg O2/g.

Executive summary:

A study was performed to determine the chemical oxygen demand of FAT 40170 according to CIBA method A-22. Based on the findings from the study, the COD of FAT 40170 is 221.1 mg O2/g

Reference 4

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

COD value

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-22

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Details on study design:

None

Preliminary study:

None

Test performance:

None

Details on results:

None

Key result

Parameter:

COD

Value:

825.9 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

no

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The COD of the test substance FAT 40170 is: 825.9 mg O2/g.

Executive summary:

A study was performed to determine the chemical oxygen demand of FAT 40170 according to CIBA method A-22. Based on the findings from the study, the COD of FAT 40170 is 825.9 mg O2/g

Reference 5

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

COD value

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

2 h

Initial conc.:

5 other: mg

Based on:

COD

Details on study design:

None

Reference substance:

other: Potassium hydrogen phthalate solution

Preliminary study:

None

Test performance:

None

Parameter:

other: COD value is available

Remarks on result:

other: COD value available

Key result

Parameter:

COD

Value:

760 mg O2/g test mat.

Results with reference substance:

The COD of the reference substance is: 200 mg O2/I

None

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The COD of the test substance FAT 40170 is: 760 mg O2/g.

Executive summary:

A predeterminated amount of the test substance FAT 40170/D dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DIN 38409 - H 41-1 (1980) corresponding to EEC Directive 92 / 69 / C.6. (1992). The test substance was tested with a sample weight of 5.0 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I.

The COD of FAT 40170/D is 760 mg O2/g

The COD of the reference substance is: 200 mg O2/I

Reference 6

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge of a communal sewage treatment plant Ara Reinach collected on 01.11.94

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Details on study design:

Experimental conditionsWater / Nutrient: according to the specifications of the OECD guidelineTemperature: 20 - 25 °C (room temperature)Test duration: 28 daysLighting: indirect daylightEstimation Technique: TOC/DOC Analyzer 500

Reference substance:

diethylene glycol

Preliminary study:

None

Test performance:

None

Key result

Parameter:

% degradation (DOC removal)

Value:

4

Sampling time:

28 d

Remarks on result:

other: Standard deviation not available

Details on results:

None

Results with reference substance:

The biodégradation of the reference substance after 8 days was: 98 %

None

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Biodegradation of the test substance after 28 days was 4 %.

Executive summary:

A key study was performed to determine the percent biodegradation of FAT 40170. An inherent biodegradability of FAT 40170 was determined in a modified Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The test substance was tested in a concentration of 150 mg/l. The reference substance (Diethylene glycol) was tested in a concentration of 150 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 4 %.

Description of key information

The test substance, Reactive Orange 13, can be considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

A key study was performed to determine the percent biodegradation of Reactive Orange 13. An inherent biodegradability of Reactive Orange 13 was determined in a modified Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The test substance was tested in a concentration of 150 mg/l. The reference substance (Diethylene glycol) was tested in a concentration of 150 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 4 %.

Another key study was performed with a predeterminated amount of the test substance dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DIN 38409 - H 41-1 (1980) corresponding to EEC Directive 92 / 69 / C.6. (1992). The test substance was tested with a sample weight of 5.0 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I.

The COD of FAT 40170/D is 760 mg 02/g. The COD of the reference substance is : 200 mg O2/I

Supporting studies were also performed to determine the chemical oxygen demand of FAT 40170 which was found to be 221.1 and 825.9 mg O2/g test material whereas the biological oxygen demand was found to be 0.0 and 2.6 mg O₂/g test material.

The relevant BOD5/COD quotient is <0.5, therefore the substance is not indicative of rapid degradation.

Taking into consideration the data from key study and supporting studies, the test substance, Reactive Orange 13, can be considered to be not biodegradable.