2-[(1-amino-9,10-dihydro-4-hydroxy-9,10-dioxo-2-anthryl)oxy]ethyl ethyl carbonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Pre-guideline test according to internal SOP (Method A-11).

Deviations:

not applicable

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

FAT 36052/E was dissolved with acetone and in parallel and in another experiment FAT 36052/E was dissolved in Tween 80.

Test organisms (species):

other: forellen (trout) as well as Red killi

Details on test organisms:

Experiment with trouts and acetone as vehicle: 8 trouts each conc were used;
Experiment with trouts and Tween 80 as vehicle: 8 trouts each conc were used;
Experiment with Red Killi and Tween 80 as vehicle: 10 fish each conc were used.

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Test temperature:

Experiments with trouts: 14 °C
Experiment with Red killi: 25 °C

pH:

7.9 - 8.1

Nominal and measured concentrations:

Experiment with trouts and acetone as vehicle: 100, 200 and 1000 mg/l
Experiment with trouts and Tween 80 as vehicle: 100, 200 and 1000 mg/l
Experiment with Red Killi and Tween 80 as vehicle: 200 and 1000 mg/l

Details on test conditions:

8 trouts were used for the experiment.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Experiment with trout and acetone as vehicle (conc used - 100, 200 and 1000 mg/l): no mortality;
Experiment with trout and Tween 80 as vehicle (conc used - 100, 200 and 1000 mg/l): no mortality;
Experiment with Red Killi and Tween 80 as vehicle (conc used - 200 and 1000 mg/l): no mortality at 200 mg/l, however 1 out of 10 fish was found dead at 48 hours with the concentration 100 mg/l.

Validity criteria fulfilled:

not specified

Conclusions:

LC50 for FAT 36052/E was found to be >1000 mg/l.

Executive summary:

The acute fish toxicity was investigated in three different experiments. In an experiment with trouts and acetone as vehicle including concentrations of 100, 200 and 1000 mg/l tested on eight touts each. No motality was observed. In a second experiment with trouts and Tween 80 as vehicle concentrations of 100, 200 and 1000 mg/l tested on 8 trouts each also resulted in no mortality. A third experiment with Red Killi and Tween 80 as vehicle involving 200 and 1000 mg/l tested on 10 fish each 1 death occured at 1000 mg/l concentration level, only. Based on these experiments, LC₅₀for FAT 36052/E was found to be >1000 mg/l.

Description of key information

The acute fish toxicity was investigated in three different experiments. In an experiment with trouts and acetone as vehicle including concentrations of 100, 200 and 1000 mg/l tested on eight touts each. No motality was observed. In a second experiment with trouts and Tween 80 as vehicle concentrations of 100, 200 and 1000 mg/l tested on 8 trouts each also resulted in no mortality. A third experiment with Red Killi and Tween 80 as vehicle involving 200 and 1000 mg/l tested on 10 fish each 1 death occured at 1000 mg/l concentration level, only. Based on these experiments, LC₅₀ for FAT 36052/E was found to be >1000 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L