3-(dimethylamino)-N,N-dimethylpropionamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-10-30 to 2018-11-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

not applicable

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control and nominal test concentration 100 mg/L
- Sampling method: Samples were taken from the control and the 100 mg/L test group from the bulk test preparation at 0 hours and from the pooled replicates at 48 hours for quantitative analysis. Samples were immediately analyzed. Duplicate sets samples were taken at 0 and 48 hours and stored frozen for further analysis if necessary.
- Sample storage conditions before analysis: Samples were immediately analyzed. Duplicate sets samples were stored frozen for further analysis if necessary.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A nominal amount of test item (100 mg) was dissolved in test water and the volume adjusted to 1 liter to give the 100 mg/L test concentration. The stock solution was inverted several times to ensure adequate mixing and homogeneity.
- Controls: negative control

- All other template details: Not reported

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): less than 24 hours
- Source: in house laboratory cultures
- Feeding during test
- Food type: mixture of algal suspension (Desmodesmus subspicatus) and GEMMA Micro 300 suspension
- Frequency: daily

- All other template details: Not reported

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

not reported

Test temperature:

20 °C to 21 °C

pH:

8.2 to 9.5

Dissolved oxygen:

8.7 to 8.8 mg O2/L

Salinity:

not applicable

Conductivity:

not reported

Nominal and measured concentrations:

Nominal test concentrations (mg/L): 100.

Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 95% to 100% of the nominal concentration and so the results are based on the nominal test concentration.

Details on test conditions:

TEST SYSTEM
- Test vessel: 150 mL glass beakers
- Material, size, headspace, fill volume: 100 mL of test preparation
- Renewal rate of test solution (frequency/flow rate): The test preparations were not renewed during the exposure period.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted Water – Elendt M7 Medium
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: not reported
- Conductivity: not reported
- Salinity: not reported
- Culture medium different from test medium: no
- Intervals of water quality measurement: Water temperature was recorded daily throughout the test. Dissolved oxygen concentrations and pH were recorded at the start and termination of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: between 200 and 1200 Lux

- All other template details: Not reported

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities:
- Other biological observations: No sub lethal effects of exposure were observed throughout the test,
- Mortality of control: No mortality occurs in the control group throughout the test.
- Other adverse effects control: none of the control daphnids sother signs of disease or stress.
- Immobilisation of control: none of the control daphnids showed immobilization
- Abnormal responses: none

Results with reference substance (positive control):

EC50 (24 hour) : 1.1 mg/L; 95% confidence limits 0.95 to 1.3 mg/L
EC50 (48 hour) : 0.80 mg/L; 95% confidence limits 0.12 to 1.5 mg/L

No immobilization or adverse reactions to exposure were observed in the daphnids exposed to a nominal test concentration of 100 mg/L for a period of 48 hours. Inspection of the immobilization data gave the following results:

Time (Hour)	EC50 (mg/L)	NOEC (mg/L)
24	>100	100
48	>100	100

Validity criteria fulfilled:

yes

Conclusions:

- The test was considered to be valid given that none of the control daphnids showed immobilization or other signs of disease or stress and that the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.
- The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 value of greater than 100 mg/L. The NOEC was 100 mg/L.

Executive summary:

A study was performed to assess the acute toxicity of the test item to Daphnia magna accroding to OECD 202. 20 daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at a nominal concentration of 100 mg/L for 48 hours at a temperature of 20 °C to 21 °C under static test conditions. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 95% to 100% of the nominal concentration and so the results are based on the nominal test concentration. Exposure of Daphnia magna to the test item gave EC50 values of greater than 100 mg/L.  The No Observed Effect Concentration (NOEC) was 100 mg/L.

Description of key information

A study was performed to assess the acute toxicity of the test item to Daphnia magna accroding to OECD 202. 20 daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at a nominal concentration of 100 mg/L for 48 hours at a temperature of 20 °C to 21 °C under static test conditions. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 95% to 100% of the nominal concentration and so the results are based on the nominal test concentration. Exposure of Daphnia magna to the test item gave EC50 values of greater than 100 mg/L.  The No Observed Effect Concentration (NOEC) was 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L