Registration Dossier
Registration Dossier
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EC number: 210-682-3 | CAS number: 621-35-2
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-acetamidoanilinium chloride
- EC Number:
- 210-682-3
- EC Name:
- 3-acetamidoanilinium chloride
- Cas Number:
- 621-35-2
- Molecular formula:
- C8H10N2O.ClH
- IUPAC Name:
- 3-acetamidoanilinium chloride
- Test material form:
- solid: bulk
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source: the aeration Stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.
- Maintainance: the sample was maintained at 21 °C with continuous aeration and used on the day of collection. The pH was buffered to pH 6.7 with sodium hydroxide solution and the suspended solids level adjusted to 4.0 g/l prior to use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- EC50 (3h): 10 mg/l
Applicant's summary and conclusion
- Conclusions:
- EC50 (3h) > 10 mg/l (nominal)
- Executive summary:
Activated sludge respiration inhibition test has been performed on test item. A range-finding investigation was conducted at the nominal concentrations of 1.0, 10, 100 and 1000 mg/l. The main test was conducted at the nominal concentration of 10 mg/l; three replicates were assayed. Blank control and reference item, 3,5-dichlorophenol, have also been tested.
The EC50 after 3 hours of contact time has been indicated as higher than 10 mg/l.
Conclusion
EC50 (3h) > 10 mg/l (nominal)
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