Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14-18 January 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: bulk

Test solutions

Details on test solutions:
Test item was direct dispersed in water.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.79 ± 0.3 cm
- Weight at study initiation: 0.86 ± 0.13 g

ACCLIMATION
- Health during acclimation: 0 % mortality in the 7 days prior to the start of the test.
- Medication: none

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes
- N. organisms per replicate: 10.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 1 replicate.
- Biomass loading rate: 0.43 g bodyweight/litre

TEST MEDIUM / WATER PARAMETERS
- Dilution water: dechlorinated tap water (Sodium thiosulphate).
- Temperature: 14 °C
- Hardness: approximately 50 mg/l as CaCO3

RANGE FINDING
- Concentration: 1.0, 10 and 100 mg/l (nominal)
- N. organisms per replicate: 2

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Any other information on results incl. tables

Main test

Concentration
(mg/l )
Cumulative mortality (initial population: 10)
24 hrs 48 hrs 72 hrs 96 hrs
Control 0 0 0 0
Test item 100 0 0 0 0
Test item 100 0 0 0 0

Applicant's summary and conclusion

Conclusions:
LC50 (96h) > 100 mg/l (nominal)
Executive summary:

The short-term toxicity to fish of the test item has been investigated using Rainbow trout (Oncorhynchus mykjss). A range-finding experiment was conducted at the nominal concentrations of 1.0, 10 and 100 mg/l. The main test was conducted at the nominal concentration of 100 mg/l; two replicates were assayed. Blank control was also been tested.

No deaths weres recorded in the main experiment replicates.

Conclusion

LC50 (96h) > 100 mg/l (nominal)