2-chloroaniline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: National guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1986

Reference 2

Reference Type:

other: national guideline

Title:

Unnamed

Year:

1980

Reference 3

Reference Type:

publication

Title:

Aquatic toxicological aspects of dithiocarbamates and related compounds. Short-term tests

Author:

Van Leeuwen CJ, Maas-Diepeveen JL, Niebeek G, Vergouw WHA, Griffioen OPS, Luyken MW

Year:

1985

Bibliographic source:

Aquat. Toxicol. 7, 145-164

Reference 4

Reference Type:

publication

Title:

A simplified method of evaluating dose-effect experiments

Author:

Litchfield JT Jr, Wilcoxon F

Year:

1949

Bibliographic source:

J. Pharmacol. Exp. Therap. 96, 99-113

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: NEN 6501 (1980) (Dutch Standardization Organization)

Deviations:

yes

Remarks:

: only "slight modifications (Van Leeuwen et al. 1985)"; The daphnids were fed on 1E8 cells/L Chlorella pyrenoidosa

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-chloroaniline

EC Number:

202-426-4

EC Name:

2-chloroaniline

Cas Number:

95-51-2

Molecular formula:

C6H6ClN

IUPAC Name:

2-chloroaniline

Details on test material:

purity >/= 99%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

isopropanol (purity 99%)

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.13 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.09-0.19 mg/L

Reported statistics and error estimates:

LC50 values and their 95% confidence intervals were calculated according to Litchfield and Wilcoxon (1949)

Any other information on results incl. tables

no further data

Applicant's summary and conclusion

Executive summary:

Maas-Diepeveen and van Leeuwen (1986)

The EC50 (48 h) for Daphnia magna was determined in a laboratory study according to the Dutch national guideline NEN 6501 of 1980 (Dutch Standardization Organization) to be 0.13 mg/L (95% CV: 0.09 -0.19 mg/L), based on test material.