Salts of 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthenium chloride and 4-{[1-hydroxy-6-({[5-hydroxy-6-(phenyldiazo)-7-sulfonaphthalen-2-yl]carbamoyl}amino)-3-sulfonaphthalen-2-yl]diazo}benzoic acid (2:1)

Endpoint summary

Additional information

Appearance/physical state/colour

The substance “Reaction product of Direct Orange 102 and Basic Red 1" as F 213 Red is a red powder at 20°C and 101.3 kPa.

Melting point

The determination of the melting point of F 213 Red was performed according to EU guideline A.1 and OECD 102 by capillary method. Using a linear heating rate of 0.5 °C/min, F 213 Red started to decompose, before the process of melting could be observed. During the test the following transformation temperatures were recorded:

1. Start of decomposition: The test item blackened.

2. 2nd step of transition: The shrunken test item changed greenish.

3. Final stage of transition: The amount of test item significantly decreased and black residue adhered to the capillary wall. No liquid phase.

F 213 Red started to decompose at a temperature of about 224 °C, i.e. 497 K.

 

Boiling point

The determination of the boiling point was performed according to EU guideline A.2 and OECD 103 also by using the capillary method. No experimental results measured during this test are reported because it does not generate new information related to the “Melting Point / Melting Range Determination of F 213 Red” study. Conclusion is made based on the observations of the melting point test and summarized in a short report according to the Sponsor’s request. In the melting point test F 213 Red started to decompose at a temperature of 224°C. No further observation was made up to 400°C (no liquid phase).

Density

The determination of the relative density was performed according to EU guideline A.3 and OECD 109 by using the air comparison pycnometer method for solids. The relative density (D420) of F 213 Red at 20°C was determined to be 1.357 ± 0.0118 g/cm2(95 % confidence interval).

Particle size distribution (Granulometry)

The particle size distribution was waived, as according to Regulation (EC) No 1907/2006 the study only needs to be conducted if the substance is marketed or used in a solid or granular form which is not the case for F 213 Red. Based on information provided by the sponsor, the substance is marketed and/or used in form of a liquid preparation.

Vapour pressure

The experimental determination of the vapour pressure was not performed as according to Regulation (EC) No 1907/2006 the study only needs to be conducted if the melting if the melting point is above 300 °C. In the study on melting point / melting range determination with F 213 Red no liquid phase was observed up to 400 °C. Instead, F 213 Red started to decompose at a temperature of 213 °C, i.e. 486 K. Thus, no study on vapour pressure measurement was conducted. Based on information provided by the sponsor, the substance F 213 Red is marketed and/or used in form of a liquid preparation.

Partition coefficient

The determination of the partition coefficient Pow was performed according to EU guideline A.8 and OECD 117 was determined based on a calibration curve using eight reference items. The capacity factors k were calculated from the dead time and retention times of the reference substances. The log k data of the reference substances were plotted against their log Pow values. The equation of the curve fitted to the calibration points is as follows: log k = 0.363 * log Pow – 0.607 (Rsq = 0.988). Using high performance liquid chromatography the partition coefficient (Pow) of F 213 Red was determined to be 2.94 ± 0.03 (95 % confidence interval), the estimated Pow 879 ± 63 (95 % confidence interval), respectively.

 

Water solubility

The determination of the partition coefficient Pow was performed according to EU guideline A.6 and OECD 105. In a preliminary test the water solubility of F 213 Red was estimated to be 0.79 mg/l and therefore the column

elution method was used in the main test. The calibration curves were prepared on each analytical occasion. The F 213 Red concentrations were calculated with the help of the calibration equations.

In the main test the water solubility of F 213 Red was determined to be 0.65 ± 0. 14 mg/l (95% confidence interval) at 20 °C ± 1 °C using the column elution method. Two parallel columns with two different flow rates were used: ca. 25 ml/hour and 12.5 ml/hour.

 

Surface tension

According to European Community Regulation 1907/2006 Annex VII, Section 7.6 the test on surface tension determination does not need to be conducted, if the water solubility is below 1 mg/l at 20°C. In the study on melting point / melting range determination with F 213 Red no liquid phase was observed up to 400°C. Instead, F 213 Red started to decompose at a temperature of 213°C, i.e. 486 K. Thus, no study on Surface Tension measurement was conducted.

Flash point

As test substance F213 Red is a solid, the determination of the flash-point is not reasonable / unjustified. Instead, the test on flammability according to the European Community Regulation 1907/2006 Annex VII, Section 7.10, was performed.

Auto flammability

The determination of the auto flammability was performed according to EU guideline A.16. No self-ignition temperature was observed up to the temperature of decomposition (determined to start at about 224°C, i.e. 497 K)

 

Flammability

F 213 Red is not flammable (as such or in contact with water) and has no pyrophoric properties. As F 213 Red is a solid, a study according to EU Method A.9 (Flash Point (liquids) or EU Method A.11 (Flammability (Gases))) is not required. Considering the chemical structure of F 213 Red there are no groups associated with flammability properties and/or pyrophoric properties, further experience in handling and use does not indicate flammability upon contact with water and/or pyrophoric properties. Thus, the experimental test to determine whether the reaction of a substance with water or damp air leads to the development of dangerous amounts of gas or gases, which may be highly flammable, according to Test Guideline A.12, as well as the determination of pyrophoric properties according to Test Guideline A.13 of EC Directive 92/69/EEC, was replaced by a theory based evaluation.

Explosiveness

The test method for the determination of the explosive properties of a substance as described for solid materials in Test Guideline A.14 of EC Directive 92/69/EEC was replaced by a theoretical based evaluation. The chemical structure of F 213 Red does not contain chemical groups or individual structural components of the molecule, which may lead to the conclusion that explosive properties have to be expected. Therefore, experimental determination of explosive properties was not performed.

Oxidising properties

The test method for the determination of the oxidising properties of a substance as described for solid materials in Test Guideline A.17 of EC Directive 92/69/EEC was replaced by a theoretical based evaluation. In general, oxidising properties are not expected for an organic molecule that does not contain oxygen, chlorine or fluorine or if the molecule does contain oxygen, chlorine or fluorine, but these elements are only chemically bonded to carbon or hydrogen. As can be seen from its chemical structure this is not the case for F 213 Red. From the two sulphur trioxides contained in the structure of F 213 Red no oxidising properties are expected, because sulphur trioxide is not oxidising. None of these oxygen atoms impose any oxidising properties on the molecule. Further, based on experience in handling and use, no oxidising properties are expected. F 213 Red is not oxidising. No experimental determination was carried out.