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EC number: 209-502-6 | CAS number: 583-39-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: A reliable publication, summarising derivative pharmaco-toxicological action, was used. However the substance of interest was not revisited in order to verify the contents; for this reason reliability score 2 was used.
Data source
Reference
- Reference Type:
- secondary source
- Title:
- 2-benzimidazolethiol
- Year:
- 2 012
- Bibliographic source:
- Japan Existing Chemical Data Base (JECDB)
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- no guideline available
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Benzimidazole-2-thiol
- EC Number:
- 209-502-6
- EC Name:
- Benzimidazole-2-thiol
- Cas Number:
- 583-39-1
- Molecular formula:
- C7H6N2S
- IUPAC Name:
- 1H-benzimidazole-2-thiol
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 5000 μg/plate (TA100);
Doses : 0, 312.5, 625, 1250, 2500, 5000 μg/plate. - Vehicle / solvent:
- 0.5% CMC
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-aminoanthracene; AF-2
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Bacterial test
Purity : 98.5%
Test species/strains : S.typhimurium TA100, TA1535, TA98, TA1537
E. coli WP2 uvrA
Test methods : Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
Procedures : Plate incorporation method
Solvent : DMSO
Positive controls : -S9 Mix, AF-2 (TA100, WP2, TA98) sodium azide (TA1535) and 9-aminoacridine (TA1537)
+S9 Mix, 2-aminoanthracene (all strains)
Doses : 0, 312.5, 625, 1250, 2500, 5000 μg/plate
S-9 : Rat liver, induced with phenobarbital and 5,6-benzoflavone
Plates/test : 3
Number of replicates : 2
GLP : Yes
Test results:
Minimum concentration of the test substance for which toxicity was observed:
Toxicity was observed at a concentration of 5000 μg/plate (TA100) with or without metabolic activation.
Genetic effects:
S. typhimurium TA100, TA1535, TA 98, TA1537
+ ? -
without metabolic activation: [ ] [ ] [*]
with metabolic activation: [ ] [ ] [*]
E. coli WP2 uvrA
without metabolic activation: [ ] [ ] [*]
with metabolic activation: [ ] [ ] [*]
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Ames tests suggest that the substance colud not be any mutagenic effects.
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