Registration Dossier
Registration Dossier
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EC number: 287-574-8 | CAS number: 85536-87-4
Endpoint summary
Administrative data
Description of key information
Biodegradation in water: screening test
The test substance is considered to be not readily / inherently biodegradable.
Additional information
Reactive Orange 72/78 displays a low ready biodegradability in that its structural analogue (SA01) achieved < 10% biodegradation in a modified OECD screening test and was not inherently biodegradable, achieving 4% biodegradation in a Zahn-Wellens test. Microbial decolourization of azo dyes in an anaerobic environment occurred as a result of reduction of azo bonds, leading to decolourized metabolites. No ready biodegradation of these metabolites was expected in the anaerobic system. However, these metabolites could be further diminished by means of either biodegradation or autoxidation under subsequent aerobic treatment. The mixed bacterial cultures decolorized three structurally dissimilar azo dyes, suggesting that anaerobic decolourization was not a specific process (Supaka 2003). It was clear that the majority of the colour removal occurred in the anaerobic stage.
The structural analogue 03, of which Reactive Orange 72/78 is a metabolite, accordingly shows no inherent biodegradation after 28 days (DOC removal) of testing, using the OECD 302B protocol. Adsorption effects (9%) were observed in the test, however the final DOC elimination after 28 days was 53%.
Based on the test result for Reactive Orange 72/78 cannot be considered as readily/inherently biodegradable; however, the substance is to some amount eliminated from the environment as shown by the 53% final DOC elimination rate of structural analogue 03.
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