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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed and documented OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dimethoxy-2-nitrobenzene
EC Number:
201-903-4
EC Name:
1,4-dimethoxy-2-nitrobenzene
Cas Number:
89-39-4
Molecular formula:
C8H9NO4
IUPAC Name:
1,4-dimethoxy-2-nitrobenzene

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
Due to the low water solubility of the test item no initial solution was prepared. The required amounts of the test substance were added directly into the testing container.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
not adapted

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
no

Test conditions

Test temperature:
20 +- 2 °C
pH:
7,2+-0,2
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
127.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
The EC50 of the positive control 3,5-Dichlorophenol was between 5 and 30 mg/L

Any other information on results incl. tables

amount test item activity respiration inhibition mean

mg/L mgO2/L*h %

1000                     36                     37.9

1000                     39                     32.8              35.3

500                       36                      37.9

500                       39               32.8              35.3

250                      43.5               25.0

250 45.0 22.4        23.7

125 45.0 22.4

125 52.5 9.5 15.9

62.5 42.0 27.6

62.5 42.0 27.6 27.6

31.3 54.0 6.9

31.3 57.0 1.7 4.3

DCP 5 58.5

DCP 20 57.0

DCP 30 58.5 58.0

DCP = 3,5 -Dichlorophenol = reference substance

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item is not harmful for activated sludge bacteria. The EC50 is greater than 1000 mg/L