Benzylamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No OECD guideline or GLP defined, acceptable restrictions (incomplete documentation).

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

other: single dermal application of undiluted test substance for 24 hours, fixed with a dressing, observation time: 14 d

GLP compliance:

not specified

Test type:

other: Acute dermal toxicity study in rats

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzylamine

EC Number:

202-854-1

EC Name:

Benzylamine

Cas Number:

100-46-9

Molecular formula:

C7H9N

IUPAC Name:

1-phenylmethanamine

Test material form:

other: liquid

Details on test material:

- Name of test material: Benzylamine

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann
- Weight at study initiation: 160 - 180 g

Administration / exposure

Type of coverage:

other: dressing (no further details)

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 h

Doses:

1.00, 1.25, 1.30, 1.50, 2.50, 5.00 g/kg bw

No. of animals per sex per dose:

1.0 g/kg bw = 10 animals/sex/dose
1.25 g/kg bw =10 animals/sex/dose
1.30 g/kg bw =10 animals/sex/dose (only male animals!)
1.50 g/kg bw =10 animals/sex/dose
2.50 g/kg bw =10 animals/sex/dose
5.00 g/kg bw = 5 animals/sex/dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: no

Statistics:

LD50 calculation according to Fink and Hund.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 1.35 other: g/kg bw

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

1.34 other: g/kg bw

Based on:

test mat.

Mortality:

5.00 g/kg bw: 5/5 males and 5/5 females;
2.50 g/kg bw: 10/10 males and 10/10 females;
1.50 g/kg bw: 10/10 males and 9/10 females;
1.30 g/kg bw: 3/10 males;
1.25 g/kg bw: 0/10 males and 2/10 females;
1.00 g/kg bw: 0/10 males and 0/10 females

Clinical signs:

other: All animals showed clinical signs after the treatment with the test substance.