Formaldehyde, polymer with benzenamine, hydrogenated

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study (GLP)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The analytical determination of the test substance concentrations was performed at the beginning and at the end of the test. The samples for analysis were taken from concentrations 70 mg.L-1 and 40 mg.L-1. All samples for analysis were delivered in transport box to analytical laboratory immediately after preparation and after end of test period (96 hours). The samples were analyzed on the day of delivery. All samples were stored at laboratory temperature.

Vehicle:

no

Details on test solutions:

Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of deionized water.
A. 117.6 g CaCl2•2H20 in 1L deionized water
B. 49.3 g MgSO4•7H2O in 1L deionized water
C. 25.9 g NaHCO3 in 1L deionized water
D. 2.3 g KCl in 1L deionized water
The dilution water should be of pH 7.8  0.2. It is saturated by oxygen by the aeration
at temperature of 20 – 24 °C. The content of sum Ca + Mg ions should have the value
of 2.5 mmol•L-1.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

supplier: EMPLA s.r.o., Hradec Kralove, (CZ)
length: 2.0 - 3.0 cm, healthy animals
Fish were acclimatized for 7 days in dilution water.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

The content of sum Ca + Mg ions should have the value of 2.5 mmol/L

Test temperature:

20 - 24 °C

pH:

7.8 +/- 0.2

Dissolved oxygen:

saturated at temparatures of 20 - 24 °C

Salinity:

freshwater

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

63 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

70 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

The sensitivity of the test species and correctness of test performance is periodically verified in six-month period by testing the reference substance, potassium dichromate.
The results of verification test with K2Cr2O7, carried out in period from 15.10. to 19.10. 2012, are the following:

48 hour – LC50 = 155 mg•L-1 (95% confidence limit: 126 – 184 mg•L-1)
96 hour – LC50 = 140 mg•L-1 (95% confidence limit: 90 – 190 mg•L-1)

Sublethal observations / clinical signs:

Nominal concentration	mg·L^-1	70	64	58	53	48	44	40	C
Number of live fish	2 hours	10	10	10	10	10	10	10	10
Mortality %	2 hours	0	0	0	0	0	0	0	0
Number of live fish	24 hours	10	10	10	10	10	10	10	10
Mortality %	24 hours	0	0	0	0	0	0	0	0
Number of live fish	48 hours	8	9	10	9	10	9	10	10
Mortality %	48 hours	20	10	0	10	0	10	0	0
Number of live fish	72 hours	3	6	9	9	10	9	10	10
Mortality %	72 hours	70	40	10	10	0	10	0	0
Number of live fish	96 hours	0	4	8	8	9	9	10	10
Mortality %	96 hours	100	60	20	20	10	10	0	0

Validity criteria fulfilled:

yes

Conclusions:

The acute fish toxicity of Formaldehyde, polymer with benzenamine, hydrogenated was determined using OECD Test Guideline No 203.
The value of LC50 (96 h) for fish (Poecilia reticulata) was determined to be 63 mg/l.

Executive summary:

The acute fish toxicity of Formaldehyde, polymer with benzenamine, hydrogenated was determined using OECD Test Guideline No 203. Test species was Poecilia reticulata. The nominal concentrations were used for all evaluations and results because evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 per cent of the nominal or measured initial concentration throughout the test.

Test validity criteria were fulfilled. The value of LC50 (96h) for fish was determined to be 63 mg/l.

 

Description of key information

The value of LC50 (96 h)  for fish was determined to be 63 mg/l. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

63 mg/L

Additional information

The acute fish toxicity of Formaldehyde, polymer with benzenamine, hydrogenated was determined according to OECD Test Guideline No 203 with Poecilia reticulata as test species. The value of the LC50 (96 h) for fish (Poecilia reticulata) was determined to be 63 mg/l.