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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
EL
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Preparation of inoculum for exposure: Sludge collected on the day of analysis. Coarse particles removed by settling for ca. 15 min, the upper layer decanted and aerated at test temperature.
- Initial biomass concentration: 1.5 g/L
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20.0 - 20.9°C
pH:
7.91 - 8.03
Nominal and measured concentrations:
10, 100, 1000 mg/L (preliminary test)
5000 mg/L (definitive test)
Details on test conditions:
TEST SYSTEM
- Test vessel: 1000 mL
- Aeration: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic sewage
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol
Duration:
3 h
Dose descriptor:
other: EC30
Effect conc.:
48.06 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: result of preliminary test
Duration:
3 h
Dose descriptor:
other: EC40
Effect conc.:
654.82 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: result of preliminary test
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: result of preliminary test
Results with reference substance (positive control):
EC50 = 15.39 mg/L (total)
EC50 = 20.45 mg/L (heterotrophic)
EC50 = 9.16 mg/L (nitrification)

Results with test substance concentration 5000 mg/L:

Total inhibition: 48.39%

Heterotrphic inhibition: 49.98%

Nitrification inhibition: 37.66%

Validity criteria fulfilled:
yes
Executive summary:

The toxicity of ASC Plus to microorganisms was tested in a respiration inhibition test according to OECD 209. The highest applied test substance concentration resulted in a total inhibition of ca. 48%. The EC50 was determined to be >5000 mg/L.

Description of key information

The toxicity of ASC Plus to microorganisms was tested in a respiration inhibition test according to OECD 209.

Key value for chemical safety assessment

EC50 for microorganisms:
5 000 mg/L

Additional information

The highest applied test substance concentration resulted in a total inhibition of ca. 48%. The EC50 was determined to be >5000 mg/L.