Methyl 2-chloropropionate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was based on EEC methodology and performed at a reputable testing facility, however no information on GLP was provided in the test report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Report states study in accordance with EEC Methodology however no specific guideline was cited.

GLP compliance:

not specified

Remarks:

No information on GLP was provided in the test report.

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-Chloropropionic acid methyl ester.

IUPAC Name:

2-Chloropropionic acid methyl ester.

Details on test material:

- Name of test material (as cited in study report): Methyl ACPA / 2-Chloropropionic acid methyl ester
- Physical state: Liquid
- Analytical purity: 99.4%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Doses:

2000 mg/kg/bw

No. of animals per sex per dose:

2 animals per sex per dose

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

not specified

Any other information on results incl. tables

A summary of the clinical signs observed during the course of the study is shown in Table 1.

 

Table1.

Signs	No of rats in group showing signs
	Male	Female
Piloerection	2/2	2/2
Hunched Posture	2/2	2/2
Waddling gait	2/2	2/2
Lethargy	2/2	2/2
Pallor of the extremities	2/2	2/2
Increased salivation	2/2	2/2
Gasping/noisy respiration	0/2	1/2
Unsteadiness	2/2	2/2
Increased respiration	2/2	2/2
Protruding eyes	1/2	1/2
Temporary loss of hind limb function	1/2	2/2

 

Applicant's summary and conclusion

Interpretation of results:

not classified

Conclusions:

The acute lethal oral dose of Methyl ACPA to rats was shown to be greater than 2000 mg/kg/bw.

Executive summary:

The oral effect of Methyl-2-chloropropionate on rats was evaluated in a study in accordance with EEC methodology. Two male and two female Sprague-Dawley rats were dosed by oral gavage at 2000 mg/kg/bw. The animals were observed and clinical signs recorded for a period of 14 days after which the animals were sacrificed.

No deaths occurred. There was evidence of systemic toxicity in all animals which persisted throughout the study. Body weight gains were considered normal for this type of study. No abnormalities were evident at the macroscopic examination.

In conclusion, the acute lethal oral dose for Methyl-2-chloropropionate was shown to be > 2000 mg/kg/bw.

The toxicity end point for the oral route of exposure is above the 2000 mg/kg/bw threshold for classification under the CLP regulation; therefore no classification relating to oral toxicity is required.