[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2-8, 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Remarks:

ISO medium, acetone

Details on test solutions:

The test solutions were prepared by using 60 μL of each WS completed to 600 mL with ISO medium.
The test solutions were then shaken for 5 minutes (300 rpm), and filtered through a 0.45 μm filter.
Filtration was needed due to the low solubility of the test item – even using a shaker and acetone as
vehicle, the formulation resulted in an emulsion, which was not appropriate for the test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Age of animals: Less than 24 h old at the beginning of the test
Sex: Female

Test type:

static

Water media type:

other: ISO medium, according to OECD 202)

Total exposure duration:

48 h

Test temperature:

20.2 and 21.1 °C

pH:

7,6

Dissolved oxygen:

> 3 mg/L

Nominal and measured concentrations:

0.007, 0.015, 0.033, 0.072, 0.159, 0.350, 0.770 mg/L.

Details on test conditions:

Based on the results of the range-finding test, a full test was conducted in order to determine the LC50
and NOEC values. Twenty Daphnids per concentration were divided into four groups and exposed for
48 hours including a renewal at 24 hours after the start. A control group also with 4 replicates run
parallel.
The applied nominal concentrations were: 0.007, 0.015, 0.033, 0.072, 0.159, 0.350, 0.770 mg/L.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate, Based on the last test, performed in May 2022, the 24-h EC50 value was determined to be 1.06 mg/L (95 % confidence limits: 1.02 – 1.10 mg/L), the 24-h NOEC: 0.50 mg/L and the 24-h LOEC: 1.00 mg/L.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 20 mg/L

95% CI:

ca. 8

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

control organisms were not immobilized

Reported statistics and error estimates:

control organisms were not immobilized

Conclusions:

In this 48-hour acute toxicity test with Daphnia magna, the 48-h EC50 based on the
geometric mean of the calculated concentrations, was determined to be 0.021 mg/L

Executive summary:

Based on the TWA concentrations of 1133-Tetrachloropropene, the 48-h EC50 was determined to be 0.022 mg/L

Description of key information

48-hour acute toxicity test with Daphnia magna,

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

ca. 20 µg/L