Fatty acids, C8-18 and C18-unsatd., zinc salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Species
:
Zebrafish, Danio rerio
Source
:
Gwangsu aquarium
Address
:
24, Gajang-ro 534 beon-gil, Osan-si, Gyeonggi-do,
Republic of Korea, 18104
Animal receipt
:
2022. 04. 12.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

48 mg CaCO3/L at 0 h, 46 mg CaCO3/L at 24 h, 49 mg CaCO3/L at 48 h and 45 mg CaCO3/L at 72 h

Test temperature:

Average, range of temperature: 21.7 ºC (21.5~22.1 ºC)

pH:

Average, range of pH: 6.73(6.50~7.12)

Dissolved oxygen:

Oxygen(D.O.): 89.2 %(74.4~106.4 %)

Salinity:

No data

Nominal and measured concentrations:

As a result of the definitive test, the mean measured concentration measured at time point 0 h, 24 h(old and new), 48 h(old and new), 72 h(old and new) and 96 h at 15.00, 24.00, 38.40, 61.44 and 98.30 mg/L were 4.06, 6.45, 10.7, 16.1 and 26.6 mg/L. These results were 27.1, 26.9, 27.9, 26.2 and 27.1 % of the nominal concentration.

Details on test conditions:

Five fish were exposed to control and 1.00, 10.00, 100.00 mg/L during an exposure period of 96 h under semi-static test. The range finding test was conducted under the same conditions of the definitive test.

Reference substance (positive control):

yes

Remarks:

Copper(II) sulfate anhydrous

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 16.824 mg/L

95% CI:

>= 14.126 - <= 21.166

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

96 h LC50: 0.089 (mg/L)
95 % Confidence limits: 0.067~0.113 (mg/L)

Validity criteria fulfilled:

yes

Conclusions:

This study evaluated acute toxicity caused by a test substance, Fatty acids, C8-18 and C18-unsatd., zinc salts in the fish(Zebrafish, Danio rerio). In accordance with the guidelines for range finding test, the treatment group received 15.00, 24.00, 38.40, 61.44 and 98.30 mg/L of test substance. The treatment and a control group remained under a semi-static test for 96 h, and then the median lethal concentration(LC50) was calculated.
During an exposure period, the mortality in the control group was not exceed 10 % and the D.O. concentration was measured at least 60 % of the air saturation value.
According to the results of range finding test, control group and 15.00, 24.00, 38.40, 61.44, 98.30 mg/L treatment groups were set. As a result of analysis of the test substance at initiation of exposure and at 24(old and new), 48(old and new), 72(old and new), 96 h, the mean measured concentration of treatment groups were 4.06, 6.45, 10.7, 16.1 and 26.6 mg/L (27.1, 26.9, 27.9, 26.2 and 27.1 % of nominal concentration). Therefore, all results were determined of the mean measured concentration.
During an exposure period, The LC50 was determined to be 16.824 mg/L(95 % confidence limits: 14.126~21.166 mg/L) at 48 h and 96 h after exposure, respectively.
The highest concentration causing no mortality was determined to be 6.45 mg/L at 48 and 96 h after exposure, respectively. And the lowest concentration causing 100 % mortality was determined to be 26.6 mg/L and 48 and 96 h after exposure.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

ca. 16.824 mg/L