Benzoic acid, 3-[[(2,3-dihydroxypropyl)amino]carbonyl]-5-nitro-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

Nov 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

rac-N-(2,3-Dihydroxypropyl)-5-nitroisophthalamic acid

EC Number:

602-890-1

Cas Number:

122731-58-2

Molecular formula:

C11 H12 N2 O7

IUPAC Name:

rac-N-(2,3-Dihydroxypropyl)-5-nitroisophthalamic acid

Sampling and analysis

Analytical monitoring:

yes

Remarks:

TOC Analysis

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
111.2 mg of ZK 39166 were dissolved in 1000 mL tap water under constant stirring for 3 hours. This solution was used as the test solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Schering AG
- Age at study initiation (mean and range, SD): 0-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

283.6 mg/L

Test temperature:

control: 20.5 - 20.1 °C
ZK 39166: 21.4 - 20.4 °C

pH:

control: 7.9 - 8.7
ZK 39166: 7.6 - 8.5

Dissolved oxygen:

control: 9.1 - 8.6 mg/L
ZK 39166: 8.4 - 8.5 mg/L

Nominal and measured concentrations:

nominal : 100 mg/L
measured: 94.8 mg/L (mean 0/48 h)

Details on test conditions:

TEST SYSTEM
20 Daphnia were used tor the test solution and also for the control group, randomly distributed into blocks of five. Labelled 100 mL test vessels were used and filled with 50 mL of the test solution and the dilution water (control group) including 5 mL of culturing water with the Daphnia, thus giving a concentration of 100 mg/L. The test was performed with 4 replicates of the test solution and the dilution water control with 5 Daphnia in each vessel. One additional test vessel was used without Daphnia for the measurements of pH, temperature and oxygen content in the dilution water control and in the test solution. The test started when the animals were added to the previously prepared test solution. The Daphnia were exposed to the test concentration and to the dilution water control for a period of 48 hours under static conditions. The light/dark rhythm was adjusted to 12 hours/12 hours.

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (48 hours) of Nip-Monoamide to Daphnia magna is higher than 100 mg/L.

Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of the compound Nip-Monoamide (ZK 39166) to Daphnia magna in a Limit-test. The study was conducted in agreement with the test guideline of the OECD, no. 202, adopted: 04 Apr. 84, and the EEC Directive 92/69, Part C2, 1992. 20 Daphnia were used tor the test solution of Nip-Monoamide and 20 for the control group. The Daphnia were exposed to the test substance and the dilution water for a period of 48 hours under static conditions in a limit-test. Immobilization was recorded at 24 hours and 48 hours. The test concentration was 100 mg/L nominal (equivalent to 94.8 mg/L measured as TOC). One immobilized Daphnia was observed in the test solution at 48 hours. Accordingly, the EC50 (48 hours) of Nip-Monoamide to Daphnia magna is higher than 100 mg/L.