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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine
EC Number:
266-719-9
EC Name:
cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine
Cas Number:
67564-91-4
Molecular formula:
C20H33NO
IUPAC Name:
(2R,6S)-4-[3-(4-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine
Specific details on test material used for the study:
Batch nr.: WF 19405; production date: 13.03.2002
purity: 100%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Municipal activated sludge from the waste water treatment plant of Mannheim/Baden-Württemberg (Germany), stored in laboratory wastewater treatment plant which was fed with municipal sewage. The sludge was washed
and aerated for 24 hours and then added to the test vessels to obtain a sludge concentration of 30 mg/l dry substance.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
< 10
Sampling time:
28 d
Details on results:
- ThOD = 2852 mg/g (calculated from analysis of the elements:
C=792 mg/g, H=109 mg/g, O=53 mg/g, N=46 mg/g);
- degree of degradation in the abiotic control: < 10%;
- degree of substance specific elimination (28 days): < 10%
(primary biodegradation);

Kinetic of test substance (in %):
ca. 0 after 7 day(s)
ca. 0 after 14 day(s)
ca. 0 after 21 day(s)
ca. 0 after 28 day(s);
Kinetic of control substance (in %):
= 60 ... 70 after 7 day(s)
= 70 ... 80 after 14 day(s)

Applicant's summary and conclusion

Interpretation of results:
other: BOD of ThOD; poorly biodegradable