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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Remarks:
BOD5 determined
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29.01.1992 - 30.03.1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
GLP compliance:
yes
Specific details on test material used for the study:
Physical appearance: liquid
Batch No.: 746916/1991
Expiry date: September 1996
Oxygen conditions:
aerobic
Inoculum or test system:
other: seeding water of an urban waste water
Duration of test (contact time):
5 d
Remarks on result:
not measured/tested
Parameter:
BOD5
Value:
38 mg O2/g test mat.
Results with reference substance:
The BOD5 of glucose-glutamic acid is 180-185 180 mg O2/l
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The BOD5 of the test substance, corrected by the blank control, is 38 mg O2/g.
Executive summary:

The BOD5 of DMPPA_701-402-5 was assessed in a study performed in accordance with the ISO 5815 Second Edition 1989-08-01 (E). Based on the findings, the BOD5 of the test substance, corrected by the blank control, is 38 mg O2/g.

Based on the results of the corresponding COD test the resulting BOD5/COD quotient is 0.03 which indicates that almost no biodegradation is likely to happen.

Endpoint:
biodegradation in water: ready biodegradability
Remarks:
Chemical Oxygen Demand
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
GLP compliance:
yes
Specific details on test material used for the study:
Test substance: DMPPA_701-402-5
Stability: guaranteed by the sponsor
Storage: room temperature
Appearance:liquid
Oxygen conditions:
other: not required as COD is being determined
Inoculum or test system:
other: not required as COD is being determined
Details on inoculum:
None
Duration of test (contact time):
2 h
Initial conc.:
2.5 other: mg
Based on:
test mat.
Reference substance:
other: Potassium hydrogen phthalate (Merck No.4876)
Preliminary study:
None
Test performance:
None
Remarks on result:
not measured/tested
Key result
Parameter:
COD
Value:
1 103 mg O2/g test mat.
Results with reference substance:
The COD of the reference substance Potassium hydrogen phthaiate is 199 mg O2/g test mat.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The COD of the test substance DMPPA_701-402-5 is 1103 mg O2/g.
Executive summary:

A study was performed to determine the chemical oxygen demand of DMPPA_701-402-5 according EEC Directive 84/449 (1984), Part. C.9 according to DEV H 41-1 Deutsche Einheitsverfahren, 1980. The COD of a test substance is calculated from the amount of the unreacted K2Cr2O7 (Merck No.4476), determinated by a titration with (NH4)Fe(SO4)2.6H2O. Based on the findings, the COD of DMPPA_701-402-5 is 1103 mg O2/g. Based on the results of the corresponding BOD test the resulting BOD5/COD quotient is 0.03 which indicates that almost no biodegradation is likely to happen.

Description of key information

The biodegradability of DMPPA_701-402-5 was assessed in studies evaluating BOD5, COD and inherent biodegradability. The BOD5 of DMPPA_701-402-5 was 38 mg O2/g, while COD was 1103 mg O2/g. Hence the BOD5/COD ratio was 0.03 (<0.5), indicating DMPPA_701-402-5 is not readily biodegradable. Two inherent biodegradability studies are available showing potential biodegradation (20-30 %), hence it can be said to fulfil the primary inherent biodegradability criteria. DMPPA_701-402-5 should not be considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, not fulfilling specific criteria
Type of water:
freshwater

Additional information