Potassium salicylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization study of sodium salicylate on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

not specified

Remarks:

Test conducted originally for other purposes than REACH and thus conducted as per the OECD guidelines only.

Test material

Test material information

Constituent 1

Reference substance name:

Sodium salicylate

EC Number:

200-198-0

EC Name:

Sodium salicylate

Cas Number:

54-21-7

Molecular formula:

C7H6O3.Na

IUPAC Name:

sodium salicylate

Test material form:

solid

Specific details on test material used for the study:

- Name of test material (as cited in study report):sodium salicylate- Molecular formula (if other than submission substance):C7H6O3.Na- Molecular weight (if other than submission substance): 160.1035- Substance type: organic- Physical state: solid- Name of test material (as cited in study report):sodium salicylate- Molecular formula (if other than submission substance):C7H6O3.Na- Molecular weight (if other than submission substance): 160.1035- Substance type: organic- Physical state: solid

Sampling and analysis

Analytical monitoring:

not required

Remarks:

As the test chemical was stable

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media and from this stock, further test solution was prepared for achieving test concentrations of 100 mg/L, respectively. ADaM’s media is used, prepared in MiliQ water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea- Source: Own breeding of daphnia magna- Food: No feeding after hatching

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Test conditions

Hardness:

145.5 mg of CaCO3

Test temperature:

20±2 °C

pH:

7.3

Details on test conditions:

TEST SYSTEM - Test vessel: Glass beaker - Aeration: No aeration during experiment - Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml - No. of organisms per vessel: 10 daphnids OTHER TEST CONDITIONS - Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Other details not known

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 100 mg/L.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical sodium salicylate. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media and from this stock, further test solution was prepared for achieving test concentrations of 100 mg/L, respectively. ADaM’s media is used, prepared in MiliQ water and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. The median effect concentration (EC50) for test chemical on Daphnia magna in a 48 hours study on the basis of immobilization effect was determine to be > 100 mg/l. Test consider to be valid as fullfills all the criteria as per standards. Firstly In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. Secondly the dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vesselsBased on the EC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.