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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Valid without restriction

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of the test substance: 505-Oximether
- Purity: 84.7 % w/w

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (SLI) was prepared by weighing of 111,0 mg test substance in 100 mL
acetone. The stock solution was stirred in acetone for approx. 1 h at 20 ± 2 0C. Ihe acetonic
stock solution (SL1) was present as olear solution.
This acetonic stock solution (SL1) was further diluted with acetone to receive stock solutions
for each test concentration (see scheme below). Ihe dilution factor was 3.
The required amount of the acetonic stock solution (10 ml *) was given into volumetric flasks
(1000 ml). After evaporating of the acetone at 200C ± 20C the volumetric flasks were filled to
1000 mL with OECD medium (1 fold concentrated). After stirring for 1 h undissolved particles
were observed in stock solutions SL2 - SL-5.
- name of the vehicle: acetone

Test organisms

Test organisms (species):
other: other algae: Desmodesmus subspicatus

Study design

Limit test:
no

Test conditions

Details on test conditions:
- Dilution water chemistry: The test medium was prepared according to OECD Guideline for Testing of Chemnicals, No. 201 Algal growth inhibition test, June 1984.
- Test temperature: 21 - 25°C (max. temperature difference 2°C) c)
- Exposure vessels: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
- Light conditions: Artificial light, permanent illumination, about 60 - 120 uE/(m2s) at a wave length of 400 - 700 nm
- Number of Replicates: 3
- Initial cell number: 10,000 cells/ml
- pH values: After 0 and 72 hours
- in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) Measurement of fluorescence: after 0, 24, 48 and 72 h
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.026 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
0.051 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.01 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

RS-Freetext:
Concentration response relationship after 72 hours:

Conc. (mg/l) / % inh. of control (biomass) / % inh. in % of control  (growth rate)
0	 /    0 / 0
0.000152 / -2.2 / -0.9
0.000457 /  4.3/ 1.4
0.00137  /  10.8/ 2.7
0.00412  /  5.1/ 0.9
0.0123   /  -5.0/ -1.9
0.0370   /  69.3/ 33.5
0.111    /  99.6/ 101.11
0.333    /  103.9/ 127.1
1        /  104.2/ 135.8

EC-values (based on mean analytically determined concentration)

EbC10 (72h) 0.0089 mg/l
EbC50 (72h) 0.0152 mg/l
EbC90 (72h) 0.0426 mg/l

ErC10 (72h) 0.0102 mg/l
ErC50 (72h) 0.0258 mg/l
ErC90 (72h) 0.0508 mg/l 

NOEC (72h) = 0.0123 mg/l (nominal)
LOEC (72h) = 0.0370 mg/l (nominal)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes