N-benzylprop-2-enamide

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 October 2007 to 23 January 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

sampling at t= 0, t=2.4 hours and t=5 days. Samples at 2.4 hours and 5 days were cooled with running tap water. All samples were diluted 100 times with 45/55 (v/v) methanol/milli-Q water and analyzed.

Buffers:

- pH: 4
- Composition of buffer: solution of 16.6% 0.05 M sodium acetate and 83.4% 0.05 M acetic acid

- pH: 7
- Composition of buffer: solution of 0.05 M potassium dihydrogenphosphate adjusted to pH 7 using 1 N sodium hydroxide

- pH: 9
- Composition of buffer: solution of 0.05 M boric acid and 0.05 M potassium chloride adjusted to pH 9 using 1 N sodium hydroxide

Details on test conditions:

TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: glass flasks
- Sterilisation method: 0.5 um filter
- Lighting: no data
- Measures taken to avoid photolytic effects: none
- Measures to exclude oxygen: nitrogen gas was purged through the flasks during 5 minutes
- No traps were used; the test system was closed
- Is there any indication of the test material adsorbing to the walls of the test apparatus: no
TEST MEDIUM
- Preparation of test solutions: 0.01 M in buffer solutions

Duration:

5 d

pH:

4

Temp.:

50 °C

Initial conc. measured:

>= 1 373 - <= 1 386 mg/L

Duration:

5 d

pH:

7

Temp.:

50 °C

Initial conc. measured:

>= 1 392 - <= 1 396 mg/L

Duration:

5 d

pH:

9

Temp.:

50 °C

Initial conc. measured:

>= 1 339 - <= 1 371 mg/L

Number of replicates:

single per pH

Positive controls:

no

Negative controls:

no

Statistical methods:

NA

Preliminary study:

only a preliminary study was performed

Transformation products:

not measured

% Recovery:

>= 99.5 - < 100

pH:

4

Temp.:

50 °C

Duration:

>= 2.4 - <= 120 h

Remarks on result:

hydrolytically stable based on preliminary test

% Recovery:

>= 99.7 - <= 100

pH:

7.1

Temp.:

50 °C

Duration:

>= 2.4 - <= 120 h

Remarks on result:

hydrolytically stable based on preliminary test

% Recovery:

>= 99.8 - <= 102

pH:

9

Temp.:

50 °C

Duration:

>= 2.4 - <= 120 h

Remarks on result:

hydrolytically stable based on preliminary test

pH:

4

Temp.:

50 °C

DT50:

> 1 yr

Type:

not specified

Remarks on result:

hydrolytically stable based on preliminary test

pH:

7.1

Temp.:

50 °C

DT50:

> 1 yr

Type:

not specified

Remarks on result:

hydrolytically stable based on preliminary test

pH:

9

Temp.:

50 °C

DT50:

> 1 yr

Type:

not specified

Remarks on result:

hydrolytically stable based on preliminary test

Details on results:

half life at 25 °C
pH 4 > 1 year
pH 7 > 1 year
pH 9 > 1 year

Conclusions:

The substance is hydrolytically stable at pH 4, 7 and 9

Executive summary:

In a hydrolysis study (preliminary test at pH, 4,7 and 9) the substance was stable over 5 days at 50 °C. The substance is considered to be hydrolytically stable at 25 °C at all pHs (t1/2 >1 year).

Description of key information

In a hydrolysis study (preliminary test at pH, 4,7 and 9) the substance was stable over 5 days at 50 °C. The substance is considered to be hydrolytically stable at 25 °C at all pHs (t1/2 >1 year).

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

at the temperature of:

25 °C