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EC number: 810-753-4 | CAS number: 1374639-78-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb - April 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9439, 1999 and ISO 10634, 1995.
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, adapted
- Details on inoculum:
- The freshly obtained sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was determined to be 4.6 g/l in the concentrated sludge. Before use, the sludge was allowed to settle (79 minutes) and the supernatant liquid was used as inoculum at the amount of 10 ml/l of mineral medium.
- Duration of test (contact time):
- > 0 - <= 28 d
- Details on study design:
- Pre-incubation medium
The day before the start of the test (day -1) mineral components, Milli-RO water (ca. 80% of final volume) and inoculum (1% of final volume) were added to each bottle. This mixture was aerated with synthetic air overnight to purge the system of CO2.
Type and number of bottles
Test suspension: containing test substance and inoculum (2 bottles).
Inoculum blank: containing only inoculum (2 bottles)
Positive control: containing reference substance and inoculum (1 bottle).
Toxicity control: containing test substance, reference substance and inoculum (1 bottle).
Preparation
At the start of the test (day 0), test and reference substance were added to the bottles containing the microbial organisms and mineral components.
The volumes of suspensions were made up to 2 litres with Milli-RO water, resulting in the mineral medium described before.
Three CO2-absorbers (bottles filled with 100 ml 0.0125 M Ba(OH)2) were connected in series to the exit air line of each test bottle. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 8
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 4
- Sampling time:
- 28 d
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of LEE011-A3 (8% and 4%).
In the toxicity control, more than 25% biodegradation occurred within 14 days (37%, based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- LEE011-A3 was designated as not readily biodegradable.
Reference
Description of key information
LEE011-A3 was designated as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
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