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EC number: 418-400-2 | CAS number: 1031-15-8 METHYL-TPP-CHLORID; MTPP-CHLORID
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan 05, 1994 - May 10, 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyltriphenylphosphonium chloride
- EC Number:
- 418-400-2
- EC Name:
- Methyltriphenylphosphonium chloride
- Cas Number:
- 1031-15-8
- Molecular formula:
- C19H18ClP
- IUPAC Name:
- methyltriphenylphosphanium chloride
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- For the preliminary test, a stock solution [A] containing 10 g/l of the test article in a solution was prepared by adding the required amount of the test article to test medium. This solution was agitated for 15 min in an ultrasonic water bath and subsequently on a magnetic stirrer. Concentration levels at or above 10.0 mg/l were achieved by dilution of the stock solution with Elendt medium by a factor of 10.
For the main test, a stock solution containing 100 mg/l of the test article was prepared as described above. The actual test concentrations were achieved by dilution of the stock solution with Elendt medium. Five concentrations between 10 mg/l and 0.6 mg/l were prepared using a serial dilution factor of 2. Additionally a concentration level of 7.5 mg/l was introduced.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Prior to testing, the daphnia were held in groups of 25-30 organisms in 1000 ml glass vessels containing synthetic water (Blendt medium). They were fed every 24 h with unicellular green algae (Scenedesmus spp.) and the water was renewed routinely on Monday, Wednesday and Friday.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- sum of calcium and magnesium ions: 2.5 mmol/L
14.5 °dH - Test temperature:
- 19.6 - 21.6°C
- pH:
- 7.5 - 8.5
- Dissolved oxygen:
- The lowest concentration of dissolved oxygen at the end of the test was 7.9 mg/L
- Salinity:
- not applicable
- Conductivity:
- 0.049 µS/cm
- Nominal and measured concentrations:
- Analytical control measurements indicated that the measured concentrations did not significantly differ from the nominal concentrations. Analysis were performed by the sponsor
(data presented in APPENDIX ill). - Details on test conditions:
- For each concentration, four 25 ml volumetric cylinders were filled with 10 ml of the appropriate test article solution. Subsequently, five daphnia were added to each test vessel.
The test vessels were closed and placed in a thermostatted incubator. The test was performed under standardized, constant conditions. In the preliminary test, the daphnia were observed after 48 h of exposure, while in the main test, observations were performed at 24 h and 48 h. In each case, two reference groups exposed to two concentration levels (0.4 and 1.4 mg/l) of the reference substance K2Cr2O7 and one control group were run concurrently.
On the basis of the results of the preliminary test, a concentration range between 10 mg/l and 0.6 mg/l was selected for the main test. The dilutions were performed stepwise by a factor of 2. A concentration level of 7.5 mg/l was used additionally. - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- In comparison to the control group, no obvious abnormal effects were seen at or below a concentration of 1.25 mg/l, which is therefore considered to represent the NOEC of the test
article. - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study.
- Reported statistics and error estimates:
- The 48 h EC50 value of 3.7 mg/l on the basis of nominal concentrations was calculated by means of Probit analysis according to Finney (1971) after log transformation of the nominal concentration values.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study a 48-hour EC50 value of 3.7 mg/L and a NOEC of 1.25 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna based on nominal exposure concentrations of the substance.
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