4-trans-ethyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008-07-22 to 2008-08-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-trans-ethyl-4'-trans-propyl-[1,1'-bicyclohexyl]

EC Number:

619-230-3

Cas Number:

96624-41-8

Molecular formula:

C17H32

IUPAC Name:

4-trans-ethyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Test material form:

other: solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

0; 96 h

Test solutions

Vehicle:

yes

Details on test solutions:

For preparation of the test solution, SPAN80 / Tween 80 (1:3 w/w) was used as solubilizer. The concentration of the vehicle was 200 mg/L.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Weight: 0.23 gLength: 3.1 cmLot: 80509

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

250 mg/L

Test temperature:

25.1 +/- 1.0 °C

pH:

According to guideline

Dissolved oxygen:

According to guideline

Salinity:

According to guideline

Nominal and measured concentrations:

57 mg/L with 100 mg/L solubiliser = (Span80:Tween 80=1:3)

Details on test conditions:

10 fish per 4 L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 57 < mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: solubiliser has been applied

Details on results:

- Mortality control: 0 % contanining the vehicle after 96 hours
57 mg/L + Solubiliser 100 mg/L: 0% (24h), 0% (48h), 0% (72h), 0%(96 h)
- Effect concentrations exceeding solubility of substance in test medium: The concentration tested was above the solubility limit of the pure test substance. To achive the exposure concentrations, solvent SPAN 80 Tween 80 = 1:3 (w/w) was used.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of the test material with solubilizer exceeds 57 mg/L, which is clearly above the limit of water solubility.

Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 203.

The LC50 of the test material with solubilizer exceeds 57 mg/L, which is clearly above the limit of water solubility.