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EC number: 947-977-8
CAS number: -
The purpose of this study was to
assess the toxicity of the test substance dissolved in fresh water, on
the reproductive efficacy of Daphnia magna STRAUS - clone 5, in a
semi-static test complying with the OECD Guideline No. 211.
The test criterion of toxicity used
was reproductive capacity expressed as the number of neonates per
daphnid per day.
The nominal concentrations used in the
study were as follows: 0, 3.25, 7.5, 15, 30 and
All concentrations given refer to the
technical product as supplied by the sponsor.
Analytical determinations of the test
solutions were made on 6 occasions during the test.
The results of the chemical analyses show
that the concentrations in the test solutions fresh and used are close
to the nominal concentration. All measured concentrations stayed within
80 to 120% of the nominal. Therefore, nominal test substance
concentrations are used to calculate the effects.
The validity criteria were respected:
No mortality occurred in the control
group over the test period.
The average number of juveniles per
parent in the control was 206after
The No Observed Effect Concentration
(NOEC) is determined as the concentration used in the study that is
immediately below the Lowest Observed Effect Concentration (LOEC), the
latter derived statistically from the data using the appropriate
were determined where possible, using statistical analysis.
weightwas found to be the most sensitive endpoint based on
An EC10for parental weight
was determined as 13.1 mg/L. The EC10for reproduction was
28.2 mg/L. Parental length gave an EC10of 32.8 mg/L with 95%
confidence limits of 3.8 to 44.9 mg/L.
Using Dunnett’s test, the Lowest
Observed Effect Concentration (LOEC) was found to be 30 mg/L for
reproduction and parental length. Based on these statistical results the
NOEC is 15 mg/L.
The EC50for parent survival
was greater than 60 mg/L.
long toxicity of Sodium diisobutylnaphthalenesulphonate to daphnia is
read across from the structurally related substance Aromatic
Hydrocarbons, C10-13, Reaction Products With Branched Nonene Sulphonated.
acute daphnia rangefinding test has been performed to evaluate which of
two very similar alkylnaphthalene sulphonates is the more toxic
substance. The results obtained indicate that there is a slightly higher
toxicity observed for batch with a higher content of longer alkyl chain
derivative when compared to the one with a higher content of shorter
observation supports the read across of the acute fish and long term
daphnia test from the source chemical Aromatic Hydrocarbons, C10-13,
Reaction Products With Branched Nonene Sulphonated, sodium salt to the
structurally strongly related target chemical Sodium
The 21d-EC10 Daphnia magna performed with the batch with
a higher content of longer alkyl chains was 6.9 mg/L in a OECD TG 211
Daphnia magna reproduction test under GLP (Reliability 1) based on
active ingredient concentration.
The toxicity of Aromatic hydrocarbons, C10-13, reaction products with
branched nonene, sulphonated, sodium salts to Daphnia magna was tested
according to OECD TG 211 Daphnia magna reproduction test. Parental
weight was the most sensitive parameter. EC10 for parental weight
was reported to be 13.1 mg/L in the 21 day test under GLP conditions.
This value equals to 6.9 mg/l based on the active ingredient
concentration. The definitive daphnia test has been performed with the
high nonene naphthalene sulphonate because algae and daphnia range
finding tests with both the low nonene and high nonene substance
indicated a slightly higher toxicity for the high nonene naphthalene
As the results of both range finding tests are considered to be valid
and because both test substances are very similar in their fate during
these tests, the conclusion that the high nonene derivative is slightly
more toxic than the low nonene derivative is considered sufficient to
select the more toxic derivative as the worst-case representative in the
hazard assessment of both substances. For this reason read-across of
ecotoxicity results of the source chemical (the high nonene derivative)
to the target chemical (the low nonene derivative) is considered to be
justified without the introduction of an additional safety factor.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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