Cyanogen bromide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Cyanogen bromide

EC Number:

208-051-2

EC Name:

Cyanogen bromide

Cas Number:

506-68-3

Molecular formula:

CBrN

IUPAC Name:

cyanogen bromide

Test material form:

solid: particulate/powder

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna

- Justification for species other than prescribed by test guideline: not relevant
- Source: Culture maintained at BRF, India.
- Age of parental stock (mean and range, SD): less than 24 h


- Feeding during test: NO

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

Range Finding: Control : 21.4 –21.5 ºC, Treatment : 21.5 – 21.9 ºC
Main Study: Control : 21.4 –21.5 ºC, Treatment : 21.4 –21.6 ºC

pH:

Range Finding: Control: 7.0 – 7.1, Treatment: 6.0 – 6.8
Main Study: Control: 7.3 – 7.4, Treatment : 6.7 – 7.3

Dissolved oxygen:

Range Finding: Control : 3.7 – 3.8 mg/l, Treatment : 3.1 – 3.5 mg/l
Main Study: Control : 3.8 – 3.9 mg/l, Treatment : 3.5 – 3.8 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): not specifiedd
- Material, size, headspace, fill volume: glass

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4




RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10, 50 and 100mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 0.038 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 0.027 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Based up on the result, Cyanogen Bromide exhibited EC50 value (95% confidence limit) of 0.0378 mg/l (Lower limit 0.0274 and Upper limit 0.0482 mg/l) in Daphnia magna for a period of 48 hour observation.

Executive summary:

Acute immobilization effect of Cyanogen Bromide Sponsored by Libor Bachura vyzkum a vyroba was tested inDaphnia magnaat Bioscience Research Foundation, India. The study was conducted as per the OECD guideline No. 202.

Solubility and Stability Analysis

The test substance 100 mg wasdissolved in 1000 ml of exposure medium and found that it was soluble. Hence exposure medium was selected as vehicle and used as control.The test substance was stable in the exposure medium for 48 hours. Hence the study was conducted as static test.

Range finding study

A range finding study was conducted using control and five different test concentrations 0.1, 1, 10, 50 and 100mg/l. Each concentration consists of four replicates and each replicate contains 5 daphnids. On the day of exposure, physiochemical parameters of the treatment and control medium were within the range. The minimum and maximum range of pH (6.0 – 7.1), temperature (21.4º C – 21.9º C) and dissolved oxygen (3.1 – 3.8 mg/l) of the control and treatment medium was recorded. At the end of 24 hour observation 0, 30, 100, 100, 100 and 100% immobility was observed in the daphnids exposed to control, 0.1, 1, 10, 50 and 100 mg/l concentrations. Lethargy was observed in daphnids exposed to 0.1 mg/l concentration. No sublethal effect was observed in daphnids exposed to control. At the end of 48 hour observation 0 and 40% immobility was observed in the daphnids exposed to control and 0.1 mg/l concentration. Lethargy was observed in daphnids exposed to 0.1 mg/l concentration. No sublethal effect was observed in daphnids exposed to control.

Main study

Based on the range finding study results, the main study was conducted with 0.003125, 0.00625, 0.0125, 0.025 and 0.05 mg/l concentrations along with control againstDaphnia magna. Each concentration consists of four replicates and each replicate contains 5 daphnids. On day ‘0’ the physiochemical parameter of the treatment and control medium was within the range. The minimum and maximum range of pH (6.7 – 7.4), temperature (21.4 º C – 21.6 º C) and dissolved oxygen (3.5 – 3.9 mg/l) of the control and treatment medium was recorded. At the end of 24 hour observation 0, 0, 0, 5, 10 and 20% immobility was observed in the daphnids exposed to control, 0.003125, 0.00625, 0.0125, 0.025 and 0.05 mg/l concentration. Lethargy was observed in daphnids exposed to 0.0125, 0.025 and 0.05 mg/l concentration. No sublethal effect was observed in daphnids exposed to control, 0.003125 and 0.00625 mg/l concentration. At the end of 48 hour observation 0, 0, 10, 20, 30 and 35% immobility was observed in the daphnids exposed to control, 0.003125, 0.00625, 0.0125, 0.025 and 0.05 mg/l concentration. Lethargy was observed in daphnids exposed to 0.0125, 0.025 and 0.05 mg/l concentration. No sublethal effect was observed in daphnids exposed to control, 0.003125 and 0.00625 mg/l concentration.

Effective Concentration

Based on the immobility result, Cyanogen Bromide exhibited EC₅₀value (95% confidence limit) of 0.0378 mg/l (Lower limit0.0274and Upper limit 0.0482 mg/l) for 48 hour exposure period.

LOEC

The Lowest Observed Effect Concentration (LOEC) was0.0274 mg/l estimated for 48 hour observation period.