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EC number: 246-325-3 | CAS number: 24577-34-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 October 2002 to 13 December 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- other: read-across target
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: In duplicate, samples of the newly prepared (clean) control medium and the newly prepared (clean) exposure medium were taken at t = 0 h and t = 48 h. Two of the same spent media were sampled again at 24 h and 72 h. 100 mL samples were collected in 125 mL Nalgene bottles. One set of samples was transferred to the analytical laboratories immediately after sampling. All other samples were stored in a freezer.
- Sample storage conditions before analysis: <-18 °C - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.3 g of test substance was weighed and transferred to an Erlenmeyer flask with a tap at the bottom. An aliquot of 3 litres of DSWL-E was added and the contents of the flask was stirred for 16 hours followed by 6 hours standing and 2 hours aeration. The aqueous phase was tapped off and the aerated for 2 hours.
- Controls: DSWL-E was used as a control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Length at study initiation : 2.7 ± 0.20 cm
- Weight at study initiation : 0.17 ± 0.046 g
- Feeding during test The fish were not fed during the test
ACCLIMATION
- Acclimation conditions (same as test or not): The fish were cultured under the same conditions as the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 209 mg/L
- Test temperature:
- 24 ± 1 °C (23.0 - 24.2 °C measured in the control aquarium)
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- Lowest value measured: 7.5 mg O2/L
- Nominal and measured concentrations:
- A water soluble fraction was prepared for the performance of the test. 0.04 - 0.18 mg/L of the test substance were measured using ICP-AES analysis.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Type : closed (covered with a watch glass)
- Material, size, headspace, fill volume: 2 L, all glass
- Aeration: The vessels were not aerated during the test
- Renewal rate of test solution (frequency): The exposure media were replaced after 24 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The groundwater was obtained from a locality near Linschoted (the Netherlands), HYDRON Medden Nederlan NV Utrecht, the Netherlands.
- Total organic carbon: 1.55 mg/L
Composition:
6.05 mg/L CO2
164 mg/L Hydrocarbon dioxide
< 2 mg/L Carbonate
<1 mg/8.03 mg/L Silicate
0.004 mg/L Manganese
0.069 mg/L Fluoride
<1 mg/L Sulphate
1.22 mmol/L Na^+
0.20 mmol/L K^+
1.38 mmol/L Ca2^2+
0.71 mmol/L Mg^2+
2.81 mmol/L Cl^-
0.65 mmol/L SO4^2-
1.39 mmol/L HCO3^-
46.25 µmol/L B
1.82 µmol/L Mn
7.22 µmol/L Li
0.59 µmol/L Rb
0.57 µmol/L Sr
0.16 µmol/L Br
0.26 µmol/L Mo
0.10 µmol/L Cu
0.10 µmol/L Zn
0.04 µmol/L Co
0.02 µmol/L I
0.01 µmol/L Se
0.05 µmol/L V
0.36 µmol /L Fe
0.67 µmol/L Na2EDTA
- Conductivity: 26.7 Ms.m^-1
- Culture medium different from test medium: Culture medium was the same used in the test
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dead and living animals were counted after 3, 24, 48, 72 and 96 hours. The condition (swimming behaviour, colour or any other visual behavioural or morphological abnormalities) were assessed at the same time as mortality observations and compared with the control animals.
TEST CONCENTRATIONS
- Range finding study: The results of the range finding test indicated that no adverse effects were expected within the water solubility of the test substance. Therefore the test was carried out using a limited test prepared as the WSF (water soluble fraction) of the test substance. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.09 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC50 of the test material is greater than the limit of solubility of the test substance. Aqueous solubility of the test material is between 0.05 and 0.18 mg/L
- Details on results:
- - Behavioural abnormalities: There were no abnormalities in the control medium or the WSF at the end of the test, and all fish were in good condition.
- Mortality of control: No mortalities occurred in the control - Sublethal observations / clinical signs:
Table 1: Results of chemical analysis
Dose
Measured Concentration mg/L
t = 0 h (clean)
t = 24 h (spent)
t = 48 h (clean)
t = 72 h (spent)
0
<0.04
<0.04
<0.04
<0.04
WSF
0.04
0.05
0.18
0.15
WSF
0.06
0.05
0.13
0.14
Table 2: Condition of the fish
Time (h)
Dose
0
WSF
0
10a
10a
3
10a
10b
24
10a
10b
48
10a
10b
72
10a
10b
96
10a
10b
a = Conditions of all fish, visually assessed, normal (=good)
b = Conditions of all fish, visually assessed, equal to that of the control fish
Table 3: Summary of results
Parameter
Effect
Dose
Measured concentration
3h LC50
Mortality
WSF
>aqueous solubility
24h LC50
Mortality
WSF
>aqueous solubility
48h LC50
Mortality
WSF
>aqueous solubility
72h LC50
Mortality
WSF
>aqueous solubility
96h LC50
Mortality
WSF
>aqueous solubility
96H LC100
Mortality
WSF
>aqueous solubility
96h NOEC
Mortality
WSF
≥aqueous solubility
96h NOEC
Condition
WSF
≥aqueous solubility
Table 4: Temperature measurements in control aquarium
Time (h)
Temperature (°C)
C
S
0
23
24
23.9
48
23
24
72
24.2
96
24.1
c = clean
s = spent
Table 5: pH and oxygen (mg/L) measurements
Time (h)
Concentration
0
WSF
pH
O2
pH
O2
C
S
C
S
C
S
C
S
0
8.1
8.7
8.1
8.6
3
7.5
24
7.9
7.9
8.6
8.0
8.2
7.9
8.6
8.2
48
8.2
7.8
8.8
7.8
8.2
7.9
8.7
8.3
72
8.2
7.9
8.7
7.9
8.2
7.9
8.8
8.3
96
7.9
7.9
7.9
8.1
c = clean
s = spent
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the test, the 96 h LC50 of the test substance in zebra fish (Brachydanio rerio), was found to be greater than the water solubility of the test substance.
- Executive summary:
The acute toxicity of the test substance to fish in Brachydanio rerio (zebra fish) was determined in a semi-static test conducted in accordance with OECD 203 and EU method C.1 and in line with GLP. Due to the insolubility of the test substance the study was performed with a water soluble fraction as a limit test. Under the conditions of the test, the 96 h LC50 of the test substance was found to be greater than the water solubility of the test substance.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted on read-across material
- Justification for type of information:
- Read-across to structurally similar substance dioctyltin oxide (Public name: DOTO, CAS: 870-08-6, EC: 212-791-1), see attached justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.09 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC50 of the test material is greater than the limit of solubility of the test substance. Aqueous solubility of the test material is between 0.05 and 0.18 mg/L
Referenceopen allclose all
Description of key information
Read-across to structurally similar substance DOTO (Dioctyltin oxide), CAS 870-08-6
Under the conditions of the test, the 96 h LC50 of the test substance was found to be greater than the water solubility of the test substance.
Key value for chemical safety assessment
Additional information
Read-across to structurally similar substance DOTO (Dioctyltin oxide), CAS 870-08-6
The acute toxicity of the test substance to fish in Brachydanio rerio (zebra fish) was determined in a semi-static test conducted in accordance with OECD 203 and EU method C.1 and in line with GLP. Due to the insolubility of the test substance the study was performed with a water soluble fraction as a limit test. Under the conditions of the test, the 96 h LC50 of the test substance was found to be greater than the water solubility of the test substance.
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