Potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctanesulphonate

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The bioaccumulation potential of the test substance was examined in and in vitro trout hepatocyte bioaccumulation screening study. Test samples were analysed up to 240 minutes.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctanesulphonate

EC Number:

261-818-3

EC Name:

Potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctanesulphonate

Cas Number:

59587-38-1

Molecular formula:

C8H5F13O3S.K

IUPAC Name:

potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctanesulphonate

Test material form:

solid: particulate/powder

Details on test material:

- Purity: 97.9%

Test solutions

Vehicle:

yes

Remarks:

Acetonitrile

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

other: In vitro study

Total exposure / uptake duration:

<= 4 h

Test conditions

Test temperature:

10-12°C

pH:

7.8

Nominal and measured concentrations:

2.33 ppm (5 μM)

Reference substance (positive control):

yes

Remarks:

4-nonylphenol

Details on estimation of bioconcentration:

Samples were examined at 5, 15, 30, 60, 120, and 240 minutes

Results and discussion

Bioaccumulation factor

Key result

Remarks on result:

other: No apparent loss of the parent compound was observed within 4 hours of incubation. Bioaccumulation potential of the test substance would not be significantly affected by fish metabolism.

Applicant's summary and conclusion

Conclusions:

No apparent loss of the parent compound was observed within 4 hours of incubation. These results suggest that the bioaccumulation potential of test substance would not be significantly affected by fish metabolism.

Executive summary:

An in vitro test was conducted to screen bioaccumulation of test substance in rainbow trout hepatocytes. Treatments consisted of test substance (final concentration of 2.33 ppm, equivalent to 5 µM), positive control and dead cell control using 2 replicates for each treatment. The hepatocyte cells of rainbow trout at a concentration of 2E6 cells/mL were used and were incubated at a temperature of 10-12°C using reaction buffer L-15 medium. The test substance was extracted in 1:9 of sample and acetonitrile and the results were analyzed using LC/MS.

No apparent loss of the parent compound was observed within 4 hours of incubation. These results suggest that the bioaccumulation potential of this compound would not be significantly affected by fish metabolism.