Formamide, N,N'-1,4-phenylenebis-, reaction products with 4-methyl-1,3-benzenediamine and sulfur, leuco derivatives

Endpoint summary

Description of key information

Short-term toxicity to invertebrates

No toxic effects of the test item were recorded up to the solubility limit of the test item in the test medium, which corresponds to 0.357 mg/L (geometric mean measured) and a nominal loading rate of 100 mg/L. Therefore, the 48 -hour EC50 and LOEC was determined to be > 0.357 mg/L (geometric mean measured).

 

Toxicity to aquatic algae

In this 72-hour growth inhibition study on algae (Raphidocelis subcapitata), the obtained results showed that the test item had not any significant toxic effects on the test system.

The 72-h NOEC was determined to be 0.126 mg/L (nominal 100 mg/L).

The 72-h LOEC was determined to be > 0.126 mg/L (nominal > 100 mg/L).

All validity criteria were met. The results are based on mean measured test item concentrations.

Additional information

Short-term toxicity to invertebrates

In the acute immobilisation test with Daphnia magna, the effects of the test item is determined according to OECD Guideline for testing of chemicals, Guideline No. 202, adopted 13th April 2004. No toxic effect up to and including aquatic saturation (i.e. limit test concentration) on Daphnia magna; the 48h LOEC is higher than the solubility level of the test item in the test medium, which corresponds to the mean measured concentration of 0.357 mg/L. The NOEC value is equal to the mean measured test item concentration of 0.357 mg/L.

Toxicity to aquatic algae

The effect of the test item Leuco Sulfur Brown 26 on the growth of a unicellular green algal species was determined in a growth inhibition study according to OECD guideline 201 and EU Method C.3.
Exponentially growing cultures of Raphidocelis subcapitata were exposed to the nominal concentration of 100 mg/L of the test item over a period of 72 hours in a limit test. Because of low solubility of the test item, test solution was prepared by utilizing the water accommodated fraction (WAF) approach. For determination of the test item concentration, samples were taken from the test concentration and control at the start and end of the test. Concentrations were determined using UV/VIS spectrophotometric method. Measured test item concentration did not remain within ± 20 % of the nominal over the test period of 72 hours, therefore the biological results are based on the mean measured test concentration.

The results obtained in this study showed that the test item Leuco Sulfur Brown 26 had not any significant toxic effects on the test system. The 72-h NOEC was determined to be 0.126 mg/L (nominal 100 mg/L). The 72-h LOEC was determined to be > 0.126 mg/L (nominal > 100 mg/L). All validity criteria were met.