Fatty acids, C16-18 (even numbered), esters with 1,2,3-propanetriol and oligomers, ethoxylated

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, C16-18 (even numbered), esters with 1,2,3-propanetriol and oligomers, ethoxylated

IUPAC Name:

Fatty acids, C16-18 (even numbered), esters with 1,2,3-propanetriol and oligomers, ethoxylated

Test material form:

solid: pellets

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was weighted in a flask, diluted with water and homogenised by ultra turrax. The solution was transferred to the test container under continouous stirring.

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

Origin: Paul Eggers, 2345 Hohenwestedt
Acclimatisation: min 14d in tapwater (20°C)
Feed: Tetra Min
length l=5.0-6.6 cm ; x=5.8 cm ; s=0.35
weight w=1.2-1.9 g ; x=1.5 g ; s=0.22 g
n=10/concentration

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

not reported

Test temperature:

Temp:20-23°C

pH:

7.5-8.3

Dissolved oxygen:

7.2-8.7 mg/L

Salinity:

not reported

Nominal and measured concentrations:

Nominal concentration: 0, 1, 10, 100, 500 mg/L

Details on test conditions:

65 hours before start of study 10 fish/concentration were placed in the test container (20l).
At test start he test substance was weighted in a flask, diluted with water and homogenised by ultra turrax. The solution was transferred to the test container under continouous stirring and pH, O2 and temperature are recorded.
Through the course of the study the behavior of the fish was observed repeatedly.
Dead fish were removed and examined macroscopical.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality occured to 2 fish in the highest concentration. No further mortality occured in all concentrations.
In the concentrations of 100 mg/L and 500 mg/L changes in behavior could be observed.
In the concentrations of 1 mg/L and 10 mg/L there was no difference in the behavior to the control.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality

Conc [mg/L]	48h		96h
	abs	rel [%]	abs	rel [%]
0	0/10	0	0/10	0
1	0/10	0	0/10	0
10	0/10	0	0/10	0
100	0/10	0	0/10	0
500	1/10	10	2/10	20

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The acute fish toxicity of the registration substance was tested in a study similar to OECD203 resulting in a NOEC (96h) of 100 mg/L and a LC50 (96h)>500mg/L nominal.

Executive summary:

The acute fish toxicity of the registration substance was tested in a study similar to OECD203 resulting in a NOEC (96h) of 100 mg/L and a LC50 (96h)>500mg/L nominal.