Reaction mass of Phosphonium, (4-ethoxy-3-methyl-4-oxo-2-butenyl)triphenyl bromide [4-Ethoxy-3-methyl-4-oxo-2-butenyl]-triphenylphosphonium bromide and Phosphonium, (4-ethoxy-3-methyl-4-oxo-2-butenyl)triphenyl chloride [4-Ethoxy-3-methyl-4-oxo-2-butenyl]-triphenylphosphonium chloride[4-Ethoxy-3-methyl-4-oxobut-2-en-1-yl](triphenyl[4-Ethoxy-3-methyl-4-oxobut-2-en-1-yl](triphenyl)phosphonium bromide

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Studiy performed according to OECD and GLP guidelines.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

Principles of method if other than guideline:

-

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of [(4-Ethoxy-3-methyl-4-oxobut-2-en-1-yl](triphenyl)-phosphonium bromide and [(4-Ethoxy-3-methyl-4-oxobut-2-en-1-yl](triphenyl)-phosphonium chloride

IUPAC Name:

Reaction mass of [(4-Ethoxy-3-methyl-4-oxobut-2-en-1-yl](triphenyl)-phosphonium bromide and [(4-Ethoxy-3-methyl-4-oxobut-2-en-1-yl](triphenyl)-phosphonium chloride

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The other eye remained untreated and served as the reference control

Duration of treatment / exposure:

-

Observation period (in vivo):

21 days

Number of animals or in vitro replicates:

3

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 21 d

Score:

ca. 11.3

Max. score:

15

Reversibility:

not reversible

Other effects:

-

Any other information on results incl. tables

-

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye)

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Substance should be classified labelled as corrosive to eyes cat.1

Executive summary:

Wittigesterhalodenid was instilled into one eye of each of three male albino rabbits, followed by seven observations at approximately 1, 24, 48 and 72 hours and 7, 14 and 21 days after test substance administration.

Under the conditions of this study, Wittigesterhalogenide resulted in adverse effects on the cornea, iris and the conjunctivae. The iridic irritation had resolved within 14-21 days, whereas the corneal injury and irritation of the conjunctivae had not resolved within 21 days after instillation in the animals. The signs of necrosis of the conjunctival tissues and the corneal injury observed during the study were considered to be corrosive effects caused by instillation of Wittigesterhalogenide in the rabbit eye.