Dimanganese trioxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

For details on the read across justification refer to the read across statement in IUCLID section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

See prinicples of method if other than guideline.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 other: % v/v saturated solution

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 other: % v/v saturated solution

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-hour LC50 of greater than 100% v/v saturated solution. Correspondingly the No Observed Effect Concentration was 100% v/v saturated solution. This study showed that there were no toxic effects at saturation.

Executive summary:

The acute toxicity of the test material to fish (Oncorhynchus mykiss) was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 203 and EU Method C.1.

As the test material is considered to be poorly soluble in water, a saturated solution of test material was prepared for use in the study.

Following a preliminary range-finding test fish were exposed, in two groups of seven, to an aqueous solution of the test material, at a single concentration of 100% v/v saturated solution for a period of 96 hours at a temperature of approximately 14ºC under semi-static test conditions. The test material solution was prepared by stirring an excess (100 mg/L) of test material in dechlorinated tap water using a magnetic stirrer at approximately 100 rpm at a temperature of approximately 14°C for 48 hours. After the stirring period any undissolved test material was removed by filtration through a 0.2 µm filter to give a saturated solution of the test material. 

The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

Two fish were observed dead in the 100% v/v saturated solution Replicate R2 after 72 hours exposure. Given that no other mortalities were observed, and no sub-lethal effects were observed throughout the duration of the test, these mortalities were observed to be due to natural causes rather than a toxic effect of the test material. There were no sub-lethal effects of exposure observed in 14 fish exposed to a test concentration of 100% v/v saturated solution for a period of 96 hours. The 96-hour LC50 value was therefore considered to be greater than 100% v/v saturated solution.

Therefore, under the conditions of the study there were no toxic effects up to the limit of solubility of the test material in the test medium.

This information is used in a read-across approach in the assessment of the target substance dimanganese trioxide.

Description of key information

Under the conditions of the valid study reported by Goodband & Mullee (2010), the acute toxicity of manganese dioxide to the freshwater fish Oncorhynchus mykiss has been investigated and gave a 96-hour LC50 value of greater than 100% v/v saturated solution. This study showed that there were no toxic effects at saturation. Correspondingly the No Observed Effect Concentration was 100% v/v saturated solution. This information is used in a read-across approach in the assessment of the target substance dimanganese trioxide.

Additional information

The information on manganese dioxide is used in a read-across approach in the assessment of dimanganese trioxide.

For details on the read across justification refer to the read across statement in IUCLID section 13.