N-cyclohexylcyclohexanamine 2-[1-[[[(1R)-1-[3-[(1E)-2-(7-chloroquinolin-2-yl)ethenyl]phenyl]-3-[2-(2-hydroxypropan-2-yl)phenyl]propyl]sulfanyl]methyl]cyclopropyl]acetate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 25th to January 28th 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Done under GLP and OECD methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1993

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: other: "Algal inhibition test" (C.3) Directive 67/548/EWG (Draft 1992), comparable to OECD TG 201

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dicyclohexylamine

EC Number:

202-980-7

EC Name:

Dicyclohexylamine

Cas Number:

101-83-7

IUPAC Name:

N-cyclohexylcyclohexanamine

Test material form:

liquid

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Purity: 99.71%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

MONITORING DATA: - cell density measurements of growth (daily)  - analysis: twice (at test start, after 72 h) 

Test solutions

Vehicle:

no

Details on test solutions:

 test concentration: 8 (0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1.0 mg/l) plus 1 control - application of test substance: mixed using an ultraturrax 8000 rpm for 1 m

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM: - stock culture: non-axenic strain obtained from the Institute of Plant Physiology at the University of Göttingen (Germany); exponentially-growing stock cultures maintained under constant temperature conditions (23 +/- 2 °C), light intensity (120 µE / m² x s); nutrient medium renewed once a week; cell density measured using a microcell counter - preculture: set up three days before test started; grown under identical exposure conditions except from the use of a different nutrient medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

pH:

- pH: 8.2 (controls at test start), 10.4 (controls after 72);  8.3 (test substance incubations at test start), 10.3-10.4 (test substance incubations after 72 h)

Details on test conditions:

Test vessel: 300 ml Erlenmeyer flasks with ground glass stoppers (closed system) - culturing apparatus: temperature 21 to 25 °C, continuous uniform illumination (spectral range 400 to 700 nm)  - light intensity: 120 µE / m² x s - test cultures: final cell density 10 E+4 cells/ml - test concentration: 8 (0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1.0 mg/l) plus 1 control - application of test substance: mixed using an ultraturrax 8000 rpm for 1 min

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.02 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.38 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.016 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.031 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

growth rate

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 0.063 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 0-72 hr ErC50 of test substance biomass was calculated to be 0.38 mg/l. The 0-72hr ErC50 of test substance growth rate was calculated to be > 1 mg/l and NOEC 0.016 mg/l.