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EC number: 276-521-4 | CAS number: 72245-24-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Benzoic acid, 2-hydroxy-, reaction products with formaldehyde, coupled with diazotized 5-amino-8-[[4-[(4-nitro-2-sulfophenyl)amino]phenyl]azo]-2-naphthalenesulfonic acid disodium salt
- EC Number:
- 276-521-4
- EC Name:
- Benzoic acid, 2-hydroxy-, reaction products with formaldehyde, coupled with diazotized 5-amino-8-[[4-[(4-nitro-2-sulfophenyl)amino]phenyl]azo]-2-naphthalenesulfonic acid disodium salt
- Cas Number:
- 72245-24-0
- Molecular formula:
- not applicable
- IUPAC Name:
- Benzoic acid, 2-hydroxy-, reaction products with formaldehyde, coupled with diazotized 5-amino-8-[[4-[(4-nitro-2-sulfophenyl)amino]phenyl]azo]-2-naphthalenesulfonic acid disodium salt
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- Han
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
-Source: KFM, CH-4414 Fullinsdorf, Switzerland
-Weight at study initiation: 160 and 190 g
-Fasting period before study: Access of food only was prevented approximately 18 hours prior and four hours after the dosing. The water bottles were withdrawn two hours prior and four hours after dosing.
-Housing: The rats were housed individually in Macrolon cages.
-Diet: standard laboratory pelleted diet (KLIBA no. 24-343-4 from Klingentalmühle AG., Basle)
-Water: ad libitum
-Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
-Temperature: 23 ± 2 °C
-Humidity: 30 - 70 %
-Air changes: 15 changes/hour
-Photoperiod: 12 hours cycle dark/light
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Amount of vehicle:
Preliminary study: 20 ml/kg body weight
Main study: 20 ml/kg per kg body weight - Doses:
- Preliminary study: 5000 mg/kg bw
Main study: 5000 mg/kg bw - No. of animals per sex per dose:
- Preliminary study: two per sex per dose
Main study: five per sex per dose - Control animals:
- no
- Details on study design:
- A preliminary study was carried out to establish a dosing regimen.
-Duration of observation period following administration: 14 days
-Observations: animals were observed soon after dosing, then at hourly intervals for the remainder day 1. On the subsequent days the animals were observed once in the morning and once in the late afternoon. Clinical signs were recorded at each observation.
The following was recorded:
1) approximate time of death;
2) the nature, severity, approximate time of onset and duration of each toxic sign
3) individual body weights on day 1, 7 and 14.
-Necropsy of survivors performed: yes
-Other examinations performed: all animals which died during the study and those killed after two weeks were subjected to a macroscopic postmortem examination. The macroscopic appearance of the abnormal organs was recorded.
Results and discussion
- Preliminary study:
- One female rat has died after exposure. After fourteen hours, during the observation period.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- - Preliminary study: all males survived; one female died
- Main study: all males survived; three females died 13 hours after dosing. - Clinical signs:
- The animals were weak, flaccid and showed a rough coat, tremors, diarrhea, hyperexitability, decreased and labored respiration, and decreased movement.
- Gross pathology:
- No special findings.
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified according to the CLP regulation (EC 1272/2008)
- Conclusions:
- LD50 (oral, rat) ca. 5000 mg/kg body weight
- Executive summary:
The acute oral toxicity was evaluated in a test performed on rats similarly to the OECD Guideline 401 method. Based on the results obtained in the preliminary study, five males and five females were dosed by oral gavage at concentration of 5000 mg/kg bw. Animals were observed for 14 days after the treatment and at the end of the study period all animals were necropsied.
The LD50 was found to be about 5000 mg/kg bw.
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