Carvacrol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from publication.

Data source

Reference

Reference Type:

publication

Title:

Report on the Safety Assessment of the given test chemical.

Author:

COSMETIC INGREDIENT REVIEW

Year:

2006

Bibliographic source:

International Journal of Toxicology

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Acute Inhalation Toxicity study of the given test chemical in rats.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

o-cresol

EC Number:

202-423-8

EC Name:

o-cresol

Cas Number:

95-48-7

Molecular formula:

C7H8O

IUPAC Name:

O-cresol

Details on test material:

- IUPAC Name: o-cresol
- Mol. formula: C7H8O
- Molecular Weight:108.139 g/mole
- InChI: 1S/C7H8O/c1-6-4-2-3-5-7(6)8/h2-5,8H,1H3
- Smiles: c1(c(cccc1)O)C
- Physical state: Solid or liquid with a phenolic odor

Test animals

Species:

rat

Strain:

other: albino rats (FDRL/Wistar stock)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: weighing between 200 and 300 g

Administration / exposure

Route of administration:

inhalation: mist

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- System of generating particulates/aerosols: The atomizer used delivered a very fine mist

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: the particle size was not stated

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

6 h

Concentrations:

0.2, 2.0, or 20.0 mg/L

No. of animals per sex per dose:

three groups of five males and five females per group

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 20 mg/L air

Based on:

test mat.

Exp. duration:

6 h

Remarks on result:

other: No mortality was observed in the study.

Mortality:

No rats died in the study.

Clinical signs:

other: Eye irritation was observed during the exposure period and these effects cleared within 24 h.

Body weight:

not specified

Gross pathology:

not specified

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Conclusions:

The acute inhalation LC50 was considered to be >20 mg/L when male and female albino rats were treated with the given test chemical via inhalation route for 6 hours exposure period.

Executive summary:

Acute inhalation toxicity study was conducted by using the given test chemical in albino rats. Thirty albino rats (FDRL/Wistar stock) weighing between 200 and 300 g were distributed into three groups of five males and five females per group. The atomizer used delivered a very fine mist but the particle size was not stated. Rats were exposed at either 0.2, 2.0, or 20.0 mg/L of aerosolized chemical for 6 h. Observations were made daily for 14 days after the exposure. No rats died in the study, but eye irritation was observed during the exposure period and these effects cleared within 24 h. Under the condition of this, the acute inhalation LC50 was considered to be >20 mg/L when male and female albino rats were treated with the given test chemical via inhalation route for 6 hours exposure period.