Reaction mass of Cobaltate(2-), [2-[[[4-hydroxy-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]phenyl]sulfonyl]amino]benzoato(3-)][2-[[2-hydroxy-5-[(phenylamino)sulfonyl]phenyl]azo]-3-oxo-N-phenylbutanamidato(2-)]-, disodium and Cobaltate(3-), bis[2-[[[4-hydroxy-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]phenyl]sulfonyl]amino]benzoato(3-)]-, trisodium and sodium bis[2-[[2-hydroxy-5-[(phenylamino)sulphonyl]phenyl]azo]-3-oxo-N-phenylbutyramidato(2-)]cobaltate(1-)

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From January the 19th to February the 07th, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Version / remarks:

2004

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Analytical method:

high-performance liquid chromatography

Type:

log Pow

Temp.:

20 °C

pH:

< 1.3

Remarks on result:

not determinable

Details on results:

MAIN TEST
Due to the fact, that the test item consist of several components, as much peaks for the test item were observed. The retention time is calculated for the each peak separately.
For all three peaks the retention time is less than the retention time of the measured reference item with the lowest partition coefficient (4-Methoxyphenol).

PRELIMINARY TEST
The preliminary test at room temperature showed, that the octanol solubility of the test item was > 379 g/l.
With the preliminary test results water solubility of > 8 g/l and the definitive test results of 14.69 g/l at 20 °C, determined in the specific study (see IUCLID section 4.8), and the solubility in octanol of > 379 g/l determined from this preliminary test, the partition coefficient was estimated to be between 1 and 1.66.

REFERENCE ITEMS

Reference item	log Pow
	1st measurement	2nd measurement
4-Methoxyphenol	1.3	1.3
Phenol	1.5	1.5
p-Cresol	1.9	1.9
4-Chlorophenol	2.4	2.4
1-Naphthol	2.7	2.7
4-Phenylphenol	3.2	3.2
2,4,6-Trichlorophenol	3.7	3.7

Conclusions:

log Pow < 1.3 at 20 °C

Executive summary:

The partition coefficient of test item was determined by HPLC method following the procedures outlined into the OECD guideline 117.

The main test was performed using formamide as dead time marker. The dead time marker was injected and measured twice before the reference items and the test item. After that two series of measurements of the reference and test items were performed.

After the measurements of the retention times tR the log k values of the reference items were plotted as a function of their log Pow values.

The partition coefficient of the test item was obtained by interpolation of the calculated capacity factor on the calibration graph of the reference items.

Due to the fact, that the test item consist of several components, as much peaks for the test item were observed. The retention time is calculated for the each peak separately. For all three peaks the retention time resulted to be less than the retention time of the measured reference item with the lowest partition coefficient (4-Methoxyphenol).

Conclusion

log Pow < 1.3 at 20 °C

Description of key information

log Pow < 1.3 at 20 °C

Additional information

OECD 117 - HPLC method