Registration Dossier
Registration Dossier
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EC number: 805-156-0 | CAS number: 137010-42-5
Endpoint summary
Administrative data
Description of key information
Glutamyl endopeptidase was tested for aquatic toxicity, including read-across studies.
Fish: Glutamyl endopeptidase has not been tested in fish, however, a similar enzyme - subtilisin - has been tested in rainbow trout for 96 hours. The 96-hour LC50 value for subtilisin was nominally 17.7 mg TOS/L, with 95% confidence limits of 13.3 and 23.5 mg TOS/L. Based on the initial measured levels, the 96-hour LC50 was 14.6 mg TOS/L (95% confidence limits, 10.6 and 19.9 mg TOS/L) corresponding to 8.2 mg aep/L. The no-observed-effect concentration (NOEC) was not clearly identified during the definitive test but based on the findings of a range finding test, the NOEC was 10 mg TOS/L (nominal) corresponding to 4.6 mg aep/L.
Invertebrates: Under the conditions of the test, testing of glutamyl endopeptidase batch PPA27269 to Daphnia magna revealed an 48h EC50 value of 5 mg TOS/L (0.7 mg aep/L). The NOEC (48h) was determined to be > 4.6 mg/L (0.66 mg aep/L).
Another similar enzyme – subtilisin – was tested using Daphnia magna. The EC value was 0.306 mg TOS/L (0.17 mg aep/L), and NOEC was 0.066 mg TOS/L (36.7 µg aep/L).
Algae: Glutamyl endopeptidase batch PPA 27269 was tested with Pseudokirchneriella subcapitata. It inhibited algal growth at 10 mg TOS/L, whereas no inhibitory effect was detected at lower concentrations. The inhibitory effect at 10 mg TOS/L was below 50% and thus no ErC values could be determined (and must be above 10 mg TOS/L (14.3 mg aep/L). The NOEC was determined to be 1 mg TOS/L (0.14 mg aep/L).
Another similar enzyme - subtilisin – was tested with Pseudokirchneriella subcapitata. The 72-hour ErC50 was 1.48 mg TOS/L corresponding to 830 µg aep/L. The NOEC was 0.566 mg TOS/L.
Additional information
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